The Role Of High Mobility Group Box 1 In The Ventilator Induced Lung Injury And Effect Of XueBiJing Injection

Objective:To explore the role of high mobility group box 1(HMGB1) in acute lung injury induced by different tidal volume of ventilation in rats and its prevention and treatment of XueBiJing injection in the ventilaor induced lung injury by detecting the expression of high mobility group box 1(HMGB1) in lung tissue.Methods:1 Twenty-four male Wistar rats were randomly divided into three groups:control group, low tidal volume group (L-VT), high tidal volume group (H-VT). The levels of myloperoxidase(MPO) activity in plasma and bronchoalveolar lavage fluid (BALF) were determined by biochemical methods respectively.Immunohistochemial staining and molecular hybridization in situ were employed to determine the expression level of HMGB1 mRNA and protein in pulmonary tissues.2 Twenty-four male Wistar rats were divided into three groups randomly:control group, H-VT group and XueBiJing pretreatment group. The levels of myloperoxidase(MPO) activity in plasma and bronchoalveolar lavage fluid (BALF) were determined by biochemical methods respectively.Immunohistochemial staining and molecular hybridization in situ were employed to determine the expression level of HMGB1 mRNA and protein in pulmonary tissues.Results:1 Comparing with control group and low tidal volume group, the levels of MPO activity in plasma and BALF were highly significant in high tidal volume group (P＜0.01); There was no statistics meaning for the differences between low tidal volume group and control group (P>0.05).2 Comparing with control group and low tidal volume group, the expression of HMGB1 mRNA and protein were highly significant (P<0.01) in high tidal volume group. There was no statistics meaning for the differences between low tidal volume group and control group (P>0.05).3 Comparing with control group, the levels of MPO activity in plasma and BALF were highly significant in high tidal volume group and XueBiJing pretreatment group(P＜0.01); Comparing with high tidal volume group, the levels of MPO activity in plasma and BALF were decreased significant in XueBiJing pretreatment group(P<0.01).4 Comparing with control group, the expression of HMGB1 mRNA and protein was highly significant in high tidal volume group and XueBiJing pretreatment group (P＜0.01); Comparing with high tidal volume group, the expression of HMGB1 mRNA and protein was decreased significant in XueBiJing pretreatment group(P<0.01).Conclusion:1 The over expression of HMGB1 mRNA and protein can be induced by high tidal volume ventilation, which can lead to increased inflammatory response in lung tissue to expand. It is one of the most important factors in VILI.2 There is small effect on the expression of HMGB1 in lung tissue in the L-VT group and no obvious lung injury.3 XueBiJing injection may play a role of prevention and treatment in the ventilator induced lung injury by inhibiting the expression of HMGB1.