The Studies To Therapy Effects On The Experimental Colitis And The Levels Of IL-23p19, IL-17A, NF-κB By Pentoxifylline

Objective: The model of experimental colitis in mice was established, and to investigate the therapy effects on the experimental colitis and the levels of IL-23p19,IL-17A,NF-κB by Pentoxifylline(PTX).Initially exploring to its possible mechanisms on the treatment of experimental colitis, and furnishing the experimental and theoretical basis to the clinic application of PTX.Methods: Healthy Kunming mice(n=38) were divided into four groups:normal group(n=8), model group(n=10), PTX group(n=10),SASP group(n=10). The experimental mice colitis model were established in model group, PTX group, SASP group by trinitrobenzene sulfonic acid(TNBS) enema local. The drug intervention was given at 24 hours after the completion of the modeling. The distilled water(0.2ml,1time/d) were given by drenching in the normal group and model group, PTX(120mg/kg,1time/d) was given by drenching in the PTX group, SASP (520mg/kg,1time/d) was given by drenching in the SASP group. Each mice of the blood in the stool, weight chang were daily recorded, the disease activity index(DAI) was evaluated. The mice in each group were killed on day 9, the occurrence of colon imflammation and ulceration, and also the pathologica changes of colonic mucosa in mice were observed. The level of IL-23p19, NF-κB of colonic mucosa were measured by ELISA, and the expression of IL-17A was evaluated by immunohistochemistry.Results: 1.The DAI, colon injury score in general morphology and histology score in model group were significantly higher than those in the normal group(P<0.05), the level of IL-23p19 , NF-κB of colonic mocosa or the expression of IL-17A in model group were also significantly higher than those in the normal group(P<0.05). Pertaining to the relationship between the level of IL-23p19 , NF-κB of colonic mucosa or the expression of IL-17A in model group with DAI, the positive correlation was found. 2.The DAI, colon injury score in general morphology and histology score in PTX group, SASP group were significantly decreased than those in the model group(P<0.05), the level of IL-23p19 , NF-κB of colonic mocosa or the expression of IL-17A in PTX group, SASP group were also significantly decreased than those in the model group(P<0.05). 3.Significan difference between PTX group with SASP group in the DAI, colon injury score in general morphology and histology score, the level of IL-23p19 , NF-κB of colonic mocosa, the expression of IL-17A were no found(P>0.05).Conclusion: It is found than PTX has a good therapeutic effection the TNBS-induced colitis in mice, the mechanisms may be the inhibition on the level of IL-23p19, NF-κB and the expression of IL-17A, thereby mitigating the inflammatory damages.