The Correlation Of T Lymphocytes Subsets And Costimulators In Patients With Type 2 Diabetes With Or Without Diabetic Nephopathy

Objective To investigate the change of T lymphocyte subsets and costimulators in peripheral blood and their roles in the development of type 2 Diabetes and diabetic nephropathy.Methods Flow cytometry was applied to detect the expression of CD3,CD4,CD8 on T lymphocyte subsets and costimulators in peripheral blood cells as well as the changes of CD4+CD28+T cells and CD4+CD154+T cells. 31 patients with type 2 diabetes without microalbminuria (DM group), 31 patients with diabetic nephropathy (DN group) and 20 healthy subjects (NC group) were investigated.Results 1,The percentage of CD4+ T cells in both DM and DN group (40.35±4.35%,42.61±3.89%) were significantly higher than that in the NC group (33.58±3.23%) (P<0.01); The percentage of CD8+ T cells in the DM and DN group (22.51±2.54%,21.2±2.57%) were lower than that in NC group (28.12±3.31%) (P<0.01); The levels of CD3+ T cells in DM and DN group (64.15±3.76%,63.87±4.54%) were lower than that in NC group (66.97±3.53%) (P<0.05); The ratio of CD4+/CD8+ in DM and DN group (1.82±0.34,2.05±0.32) were higher than that in NC group (1.21±0.17) (P<0.01); CD4+ T cells in DN group were more than that in DM group (P<0.05); CD8+ T cells and CD3+ T cells in DN group were less than that in DM group but there were no significant difference (P>0.05); the ratio of CD4+ /CD8+ in DN group was higher than that in DM group (P<0.05).2,The levels of CD3+CD28+T cells in DM and DN group (52.11±7.63%,52.57±8.04%) were higher than that in NC group (47.74±4.49%) (P<0.05); The level of CD3+CD28+T cells in DN group were higher than that in DM group, but there was no significant difference (P>0.05); The levels of CD4+CD28+T cells in DM and DN group (31.20±8.26%, 34.83±6.13%) were significantly higher than that in NC group (26.49±2.80%) (P<0.05); The level of CD4+CD28+T cells in DN group was higher than that in DM group (P<0.05).3,The levels of CD4+CD154+T cells in DM and DN group (1.733±0.57%,2.77±0.52%) were significantly higher than that in NC group (0.612±0.24%) (P<0.01); The level of CD4+CD154+T cells in DN group was significantly higher than that in DM group (P<0.01).Conclusions 1,The imbalance of T lymphocyte subsets was discovered in T2DM patients. It is more serious in patients with diabetic nephropathy. These observations suggest that abnormal regulation of autoimmune may participate in the development and progression of DM and DN.2,The levels of CD3+CD28+T cells and CD4+CD28+T cells in patients with type 2 diabetes and patients with diabetic nephropathy were significantly higher than that in healthy subjects, The level of CD4+CD28+ T cells in patients with diabetic nephropathy was higher than those without nephropathy, which suggested that the abnormal T cell activation existed in T2DM and in T2DM with diabetic nephropathy, costimulatory molecules may be involved in this abnormal immune regulation.3,The levels of CD4+CD154+T cells in patients both with type 2 diabetes and with diabetic nephropathy were significantly higher than that in healthy subjects and the level of CD4+CD154+ T cells in patients with diabetic nephropathy were higher than that in patients with type 2 diabetes, These finding indicated that CD4+CD154+T cells may be related with type 2 diabetes and diabetic nephropathy.