Expression Of RANKL In The Permanent Teeth Germ Missing Deciduous Teeth And During Different Stages Of Physiological Root Resorption Of Human Primary Teeth

Objective:To inverstigate the expression of RANKL in the permanent teeth germ missing deciduous teeth and during different stages of physio-logical root resorption of human primary teeth.Methods:Collected the physiological root resorption of human primary teeth and divided them into 4 groups:the permanent teeth germ missing deciduous tooth,early primary tooth root resorption, medium deciduous root resorption and the end of primary teeth root resorption, take the orthodontic needing extraction normal permanent as the control group.Detected the RANKL expression of the primary teeth root resorption in Immunohistochemistry and in situ hybridization. Using Image-pro-plus6 image analysis software on each set of experiments sections stained cells broken teeth, dental pulp cells, fibroblast cells accumulated optical density value (integral optical density, IOD) measured. Using One-way ANOVA and q-test (a= 0.05) were statistically analyzed.Results:1. Immunohistochemistry results:Fibroblasts accumulated in the optical density of RANKL compared with the control group than in permanent teeth and the other 4 groups, there was significant statistical difference (P<0.01). Retention of deciduous teeth root resorption of deciduous teeth group and no significant differences between the early group (P>0.05). Root resorption of deciduous teeth of early group, absorption absorption of the medium-term group was lower than the late group, there was a significant difference (P<0.01).Odontoblast cumulative optical density compared with the control group than in permanent teeth and the other 4 groups, there was significant statistical difference; stranded optical density of deciduous group cumulative root resorption of deciduous teeth of early group with no significant difference (P>0.05). Root resorption of deciduous teeth of early group, absorbing the end of the medium-term group compared with the absorption of significant statistical difference (P<0.01).No broken teeth in the permanent group of cells exists, the cumulative retention group optical density of deciduous teeth root resorption with no significant differences between the early group (P>0.05). Root resorption of deciduous teeth of early group, absorbing the end of the medium-term group compared with the absorption of a significant statistical difference (P<0.01)2. In situ hybridization resultsCompared with the RANKL protein, RANKL mRNA expression was not obvious. Fibroblasts accumulated optical density of RANKL compared to permanent teeth is far lower than the control group with the other four groups, there was significant statistical difference (P<0.01). Retention of deciduous teeth root resorption of deciduous teeth group and no significant differences between the early group (P>0.05). Root resorption of deciduous teeth of early group, absorbing medium group were lower than the absorption end of the group, there was a significant difference (P<0.01).Odontoblast cumulative optical density compared with control group and the remaining permanent teeth group teeth root absorption of early groups were significantly different (P<0.05); retention of deciduous teeth group optical density value of the cumulative absorption of primary teeth in early group no significant difference (P>0.05). Root resorption of deciduous teeth of early group, absorption absorption of the medium-term group was lower than the late group, there was a significant difference (P<0.01).No odontoclast in the permanent group of cells exists, the cumulative retention group optical density of deciduous teeth root resorption with no significant differences between the early group (P>0.05). Root resorption of deciduous teeth of early group, absorbing the end of the medium-term group compared with the absorption of a significant statistical difference (P<0.01) Conclusion:1.RANKL root resorption of deciduous teeth in different periods there were significant differences in content.2.RANKL in the absence of permanent teeth in the endosperm and a higher content of the activity is caused by deciduous teeth slow absorption of one of the factors.