MRNA Expression Of P53 In Human Brain Tumor Tissue

Objective:A glioma is a type of tumor that starts in the brain or spine. It is called a glioma because it arises from glial cells. The most common site of gliomas is the brain.It is the most common tumor in central nervous system, about count 44% of central nervous system tumor. Gliomas cannot be cured. The prognosis for patients with high-grade gliomas is generally poor, and is especially so for older patients. Of 10,000 patients diagnosed each year with malignant gliomas, about half are alive 1 year after diagnosis, and 25% after two years. Those with anaplastic astrocytoma survive about three years. Glioblastoma multiforme has a worse prognosis with less than 12 month survival after diagnosis. We choose 100 glioma cases in Dalian Medical University second affiliated hospital neurosurgery department to study the mRNA expressin of P53 in human brain tumors.Methods:mRNA of p53 gene was detected using RT-PCR in 100 cases of human gliomas,30 cases of benign brain tumors.Results:lower expression of p53 mRNA in all of brain gliomas were found compared with those in benign brain tumors(P<0.01).Conclusions:low expression of p53 mRNA may be related to the carcinogenesis and development of brain tumors and their malignant degree.