The Experimental Study Of Yuye Detoxification Particle Anti-respiratory Virus In Vitro And Anti-influenza A Virus In Vivo

Literature ResearchRespiratory Viral Infection is a kind of the most common acute respiratory disease. It can betide in every age stages. Many virus cause respiratory diseases. And it contain many kinds of diseases. The sserious clinical manifestation are bronchiolitis and pneumonia. It can caused acute respiratory distress and cardiac failure even lead to dead. Especially respiratory virus often product variation and increase pathogenicity, or lead new diseases. For example, in recent years, SARS, human avain influenza, influenza A virus have damaged people's health and lifes. While it even effect stability of social and national economy. So many people pay attation to it.This paper begins at the structure of four respiratory virus. It described the replication of four virus, epidemiolog features, Pathogenic mechanism, clinical manifestation, diagnosis and the research progress of influenza vaccine. On research of pharmaceutical anti-respiratory virus, this paper described different mechanisms and drugs between traditional chinese medicine and western medicine. And furthermore showed that traditional chinese medicine has great advantages.Experiment Study1. Object:To investigate the effect of Yuye detoxification particle anti-respiratory virus in vivo and vitro, also anti-influenza A virus in vivo.2. Method:In vitro, at first, study the influences that Yuye detoxification particle inhibitory influenza A Virus by culture MDCK. The second, study the influences that Yuye detoxification particle inhibitory Rhinovims and Adenovirus by culture HEL cells. The third.study the influences that Yuye detoxification particle inhibitory respiratory syscytial virus by culture A549 cells. In vivo, we observed the effect of the mice lung index,the death rate of mice, TNF-a, IL-10, Neuraminidase and Nucleic acid content what the Yuye detoxification particle do.We use influenza A virus drop in the mice nasal cavity.3. Result:3.1 Experiments in vitro:At the first, we use egg inoculation amplificate virus and detect the virulence by blood agglutination experiment and plaque forming assay. According to the result of blood agglutination experiment, we calculate the TCID50 is 3.67. In order to decide Yuye detoxification particle' s maximum of no toxicity concentration, we divided it into different concentration to test. And then observed the preventive effect, protective effect, direct cytotoxic effect and the dynamic of prevent virus replicate to research the effect of Yuye detoxification particle anti-influenza A virus. The result showed:Yuye detoxification particle has the effects at concentration of 125g/L and reduced two virus agglutination titer. Yuye detoxification particle inhibit Rhinovims (TCID5=5.5) and Adenovirus (TCID5= 5.67) at concentration of12.5 g/L and 6.25 g/L. The therapeutic index are 17.99 and 46.89 Yuye detoxification particle inhibit respiratory syscytial virus (TCID5=5.0) at concentration of 3.12g/L.3.2 Experiments in vivo:Yuye detoxification particle at the high dose of 15g/kg can reduced the mice lung index, alleviated pulmonary consolidation, improved the symptoms that caused of viral pneumonia, raised the mice survival rate, reduced the content of TNF-a and improved the content of IL-10. The result showed that this drug can raise the mice's phylactic power and improve immune dysfunction that caused of virus infection.It can not reduced the content of neuraminidase(NA) and showed the effect target was not NA. The result of fluorescence Polymerase Chain Reaction(PCR) indicated that Yuye detoxifi-cation particle can inhibit the mice lung tissue virus that infected from influenza A virus. According to this inhibitory effection, it can alleviate mice body injury.ConclusionThe results of this experimental research demonstrated that Yuye detoxifi-cation particle has better inhibitory effection in anti-influenza A virus research at concentration of 125g/L in vitro.And it showed different kinds of anti-virus effections.It can inhibitRhinovims and Adenovirus at concentration of 12.5 g/L and 6.25g/L. Also inhibit respiratory syscytial virus at concentration of 3.12g/L. While in vivo experiments, influenza A virus can be suppressed at the high dose of 15g/kg. At this concentration, it reduced the symptoms of viral pneumonia, prolong the mice's life. In a word, Yuye detoxification particle not only has good therapeutic effects in vitro, but also is an effective traditional chinese medicine anti-influenza virus in vivo and provide reliable laboratory basis for the clinical widely applied. But the effection of Yuye detoxification particle anti the other respiratory virus in vivo have to be proved.