| Objective:In this paper, different diameter Dragon's blood preparation from nanometer to micrometer was prepared by changing the auxiliary,solvent,temperature and other factors. The diameter of the preparations were measured by the Malvern laser size analyzer and electron microscope. After Dragon's blood preparation with different diameter were given to mice ig, its bioavailability would be texted with the balance research method.This paper would text its correlation between Dragon's blood diameter and its absorption,select the optimal diameter of Dragon's blood preparation to enhance its bioavailability,improve its efficacy and overcome Dragon's blood's poor water-solubility,improve its bioavailability in clinical.Method:The study of Dragon's blood preparation with different diameter:Dragon's blood nanometer suspension,taking 50%.60%.70%.80%.90%.100% ethanol as the main dispersion matrix system, was made up.Observed its influence to diameter by adding different type or quantity PEG to the Dragon's blood nanometer suspension taking ethanol as the main dispersion matrix system; Dragon's blood solid dispersion with different molecular weight's PEG as carrier was prepared,and measured its diameter; Dragon's blood solid dispersion with poloxamer as carrier was prepared,and measured its diameter; Dragon's blood solid dispersion with PEG and poloxamer mixtures of different ratios as carrier was prepared, and measured its diameter, examined the influence to the diameter of different ratios; Examined the influence of drug loading to the Dragon's blood diameter; Examined the influence of watering quantity to the Dragon's blood diameter; Examined the influence of tempeature to the Dragon's blood diameter.Dragon's blood preparation of 7 different diameter were selected,and studied its absorption in mice with the balance research method. Loureirin A. B and other fingerprint peak levels in mice feces were determined by HPLC-electrochemical determination(HPLC-ECD),compared its correlation between Dragon's blood diameter and its absorption.Result:The results of the prepared of different diameter Dragon's blood:The Dragon's blood nanometer suspension's diameter with different concerntrations of ethanol as the main dispersion matrix system was about 200nm in room temperature, the diameter was not influence of ethanol concerntration. There was auxiliary PEG adding to the Dragon's blood nanometer suspension with ethanol as the main dispersion matrix system, when the low temperature, adding PEG would cause less precipitation and more uniform diameter in solution, when at room temperature,there was no precipition in solution,the diameter was increasing with the adding PEG molecular increased. The diameter was about 300nm when adding PEG600, the diameter was about 2μm when adding PEG4000. The average diameter of Dragon's blood solid dispersion with PEG as carrier was about 183nm,measured by electron microscope. The diameter did not change regulary with PEG molecular weight change. Poloxamer as carrier to get Dragon's blood solid dispersion, the solution made of clarity, may form micelle. There was Dragon's blood solid dispersion with the mixture of poloxamer and PEG4000 as carrier, when the ratio of poloxamer was more than 50%,the solution was clarity, when the ratio was PEG4000:poloxamer=60:40,70:30,75:25,80:20, the average diameter was about 29,45, 59,123nm,namely along with PEG the percentage content was larger, the diameter was larger in certain rang. The carrier carried the drug was certain, when prepared Dragon's blood solid dispersion, generally 1g carrier can support 0.2g drug at most; The watering quantity did not influence Dragon's blood diameter; Temperature effected on the diameter of Dragon's blood preparation, when temperature was lower than room temperature, precipitates of different size often appeared in solution.This experiment selected Dragon's blood preparation of 7 different diameter, studied its absorption in mice with the balance research method. Loureirin A,B and other fingerprint peak levels in mice feces were determined by HPLC-ECD. The results showed that, Loureirin A,B absorption percentage in the sample that average diameter was smaller than 2.6μm was twice than in the sample that average diameter was about 100.83μm.Other fingerprint peak's absorption percentage also increased with the diameter decreased. From 2.6μm to100.83μm, the performance was most obvious with diameter decreased, the absorption rat increased. When the diameter was less than 2.6μm, futher reduce the diameter of Dragon's blood preparation,then a less extent increase in absorption rate.Conclusion:Diameter of the Dragon's blood preparation was key to its absorption. When the diameter was more than 2.6μm, the smaller the diameter, the greater percentage of Dragon's blood absorption. When the diameter is less than 2.6μm, the absorption percentage increases scope is small, the absorption possibility already achieved saturated. Absorption rate was correlated with the diameter, the smaller the diameter, the absorption rate is higher. This paper showed that, the bioavailability increased significantly after made small diameter of Dragon's blood. There was certain significance to the formulation reform of Dragon's blood.
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