Cyclosporin A Affects The Level Of Anti-myelin Basic Protein In Serum And Myelinoclasis After The Rat Brain Trauma

Objective:To investigate the effect of cyclosporin A (CsA) on the level of anti-myelin basic protein (anti-MBP) in the serum and myelinoclasis in rat models after brain trauma.Methods:Seventy-seven SD rats were randomized into blank control group (n=7), model group (n=35) and CsA-treated group (n=35). The 0.9% normal saline (5 mg/kg/d) was intraperitoneally injected into the model group and 5 mg/mL CsA (5 mg/kg/d) was injected into the CsA-treated group. Different time points (1,4,10,20 and 30 d) after the brain trauma, the MBP content and anti-MBP titer in the serum were measured using enzyme-linked immunosorbent assay (ELISA) and the degree of myelinoclasis in the brain stem slices was assessed with Marchi's method.Results:As compared with that in the blank control group, the MBP and Anti-MBP contents in the model group were significantly increased (P＜0.05):anti-MBP in the serum and the degree of myelinoclasis in the brain stem were all significantly increased in the 4th d, both reaching their highest levels on 10th d. No significant differences of the MBP content in the serum between the CsA-treated group and the model group were found the same time points after the injury (P>0.05). However, lower level of anti-MBP in the serum 1 d after the injury and degree of myelinoclasis in the brain stem 4 d after the injury in the CsA-treated group were observed as compared with those in the model group (P<0.05). Statistical analysis showed an intimate positive correlation between anti-MBP titer in serum and the degree of myelinoclasis (r=0.959, P=0.000).Conclusions:Cs A can affect the immune system to decrease the degree of myelin-oclasis in the brain stem through decreasing the level of anti-MBP in the serum of rat models after brain trauma.