Research Of Sample Handling In The Immunological Detection Against Ion Cadm Ium And Ion Lead

Objectives:To standardized the sample processing against cadmium and lead, to establish a method of sample handling and Enrichment for the Immunological detection against ion cadm ium and lead, and make stable and reliable Colloidal Gold test papers.Methods:First of all,samples must be collected including water, plants,soil and primary pro ducts,and choose different qualification to handle different kinds of samples. Then,it could using Nanometer-size TiO2 to adsorp the ion cadmium and lead,as well as explore the best conditions of Adsorption. Finally, colloidal gold immunochromatographic assay was used for detecting ion cadmium and lead, and the best conditions of Immunological detection was fixed.Results:At pH 9.0, Cd was retained on the nanometer-size TiO2, then eluted with 0.1 mol/ L EDTA·2Na quantitatively. The adsorption capacity of nanometer TiO2 was found to be 14.7 mg/g for Cd(II). The maximum preconcentration factor was 50. We made a kind of dipstick wit h colorimetric assay, nano colloidal gold particles were prepared and labeled to an anti-Cd(Ⅱ)-i EDTA (Isothiocyanobenzyi-EDTA) monoclonal antibody. Linked with nanometer TiO2 as soli d adsorbent, the whole detection process could be performed in 90 minutes, and the minimum de tection concentration could be down to 5μg/L. Samely, Pb was retained on the nanometer-size Ti 02, then eluted with 0.5 mol/L DTPA quantitatively. The maximum preconcentration factor also was 50. The method was applied to the determination of Cd (Ⅱ) and Pb(Ⅱ) in environmental wa ter samples and satisfactory results were obtained.Conclusion:A new method was developed for the rapid determination of trace Cd(Ⅱ) and Pb(II) using nanometer sized TiO2 as solid adsorbent and detected by Colloidal gold immunoch romatographic method. The sensitivity of the method is high enough to satisfy the safety require ments for non-environmental pollution aquatic products.