Application Of Real-time PCR To Investigate The Expression Level Of GYPC In Children With Acute Lymphoblastic Leukemia At Different Stages

ObjectiveThe incidence of Leukemia is highest than other malignant tumors in children. More than 90% leukemia in children is acute, The majority of acute lymphoblastic leukemia, accounting for about 70% of acute leukemia, This disease was a serious threat to the lives of the children. At the moment, the pathogeny of the ALL is not fully clear. The relevant literatures of the domestic put forward that there is a relationship between the gene of GYPC and the occurrence,the development,the prognosis of the ALL in children. This research is to investigate the expression level of GYPC in children with ALL at different stages by applying the Real-time PCR, so to ascertain the relationship between the GYPC and the ALL In children, and the relevance between the expression level of GYPC and age,leukocyte whether or not has statistical significance.Object of study and Methods1. Object of study:The research included the patients with acute lymphoblastic leukemia who came from the pediatric hematology department of the second affiliated hospital of china medical university.Also included the normal children.(1)Newly diagnosed patients with ALL:Diagnosis by bone marrow puncturing, According to the FAB diagnostic criteria (including B T series), before the treatment.(2)The remission group:The bone marrow gets complete remission (archeaocyte+ immature cells＜5%), There is no immature cells in the hemogram and the cerebrospinal fluid, the hemogram was normal, pre-chemotherapy for the next time.(3)Normal children:The patients came from the Pediatric Surgery of the shengjing hospital, who have a fracture one year ago, before taking the fixation.2. Methods:Real-time PCR was employed to investigate the expression level of GYPC in the peripheral blood in children with ALL at different stage and normal controls.3. Statistical Methods:Application of SPSS 13.0 to analyze all the data, Kruskal-Wallis test was employed to compare the multiple sets of samples,and MAann-Whitney test was used for pairwise comparision, segmentation of the alpha using bonferrinoi, P＜0.05 as statistically significant difference. Application of Spearman rank correlation to analyse the relevance between the expression level of GYPC with age,leukocyte whether or not has statistical significantce at the same time.ResultsThe expression level of GYPC in newly diagnosed ALL patients,remission group and the normal controls is different. And this difference has statistical significance (x2=13.497, P＜0.001). The expression level of the newly diagnosed group is higher than the remission group (P＜0.001) and the normal controls(P＜0.001). But the difference between the remission group and the normal controls has no statistical significance (P>0.05). The relevance between the expression level of GYPC and age has no statistical significance(correlation coefficient=-0.03,P=0.11) and the relevance between the expression level of GYPC and leukocyte also has no statistical significance (correlation coefficient=0.152,P=0.429).ConclusionThe expression level of GYPC was different at the different stage of the children with the acute lymphoblastic leukemia. The expression level of GYPC of the newly diagnosed patients was higher than the remission group(P＜0.001) and the normal controls(P＜0.001). There was no difference of the expression level of GYPC between the remission group and the normal controls (P＞0.05). The relevance between the expression level of GYPC and age,leukocyte has no statistical significanc,(P＞0.05) at the same time. That is, the expression level of GYPC in peripheral blood in children with ALL varied with the different stages. GYPC can be used as a new indicator to monitor the disease progression, predict replace and improve chemotherapy, and the specific mechanism needs further explore.