Expression And Clinical Significance Of KAI1, Tiam1, XAGE-1b In Adenoid Cystic Carcinoma

ObjectiveAdenoid cystic carcinoma of the transfer is more than one factor involved, many steps to complete the complex process, subject to the regulation of many genes and their products, including:tumor metastasis gene, tumor metastasis suppressor gene, and other genes associated with metastasis. Purpose of this study is adenoid cystic carcinoma patients by retrospective studies to clarify the prognosis of ACC and three kinds of transfer genes KAI1, Tiam1, XAGE-lb relevance for the prognosis and to provide experimental basis for clinical targeted therapy.Method39 cases of clinically collected specimens of adenoid cystic carcinoma,12 cases of parotid gland and ACC-2, ACC-M cells by histological staining of tissue and ACC cells in HE staining, tissue and ACC Immunohistochemistry stain (SABC), antibody to KAI1, Tiaml, XAGE-lb, by image acquisition, image analysis, by different research optical density between samples. Calculated by comparing the different studies and samples of the difference between the optical density analysis the correlation between each other.Using SPSS 13.0 software for statistical analysis. Statistics KAI1, Tiaml, XAGE-lb protein expression in the MOD value of quantitative indicators, with the KS test samples in each group normality, Levene test group check the homogeneity of variance, comparison by t test, P<0.05 as statistically significant difference. Each other to pearson correlation analysis. ResultsCheck each set of data, are approximate normal distribution, Levene homogeneity of variance test with each set of data.KAI1 expression:in the control parotid gland tissue MOD value is greater than the ACC, P<0.01, statistically significant difference between the two tips; in three repeated experiments in the ACC-2 of the MOD values were greater than the ACC-M, P< 0.05, suggesting that the difference was statistically significant. KAI1 protein expression and gender, age, and whether there was no significant lymph node metastasis, and TNM staging and tumor size was significantly related:TNM stage wasⅠ-Ⅱof the KAI1 protein was significantly higher than that ofⅢ-Ⅵ; tumor diameter≤4cm of KAI1 protein was significantly higher than those> 4cm's.Tiaml expression:in the control parotid gland tissue MOD value is less than ACC, P<0.01, statistically significant difference between the two tips; in three repeated experiments in the ACC-2 of the MOD values were less than ACC-M, P< 0.05, suggesting that there are differences. Tiaml protein expression and gender, age, and whether there was no significant lymph node metastasis, and TNM staging and tumor size was significantly related:TNM stage wasⅢ-Ⅵof the Tiaml protein was significantly higher than theⅠ-Ⅱperiod; tumor diameter> 4cm of Tiaml protein expression was significantly higher than the≤4cm's.XAGE-lb expression:in the control parotid gland tissue MOD value is less than ACC, P<0.01, statistically significant difference between the two tips; in three repeated experiments in the ACC-2 of the MOD values were less than ACC-M, P<0.05, statistically significant difference between the two tips. XAGE-lb protein expression and gender, age, and whether there was no significant lymph node metastasis, and TNM staging and tumor size was significantly related:TNM stage wasⅢ-Ⅵof the XAGE-1b protein was significantly higher than theⅠ-Ⅱperiod; tumor size> 4cm in XAGE-1b protein was significantly higher than the≤4cm's.The correlation coefficient of KAI1 and Tiaml r=-0.429, bilateral Pearson test P =0.006 (P<0.01), statistically significant, visible, KAI1 a high negative correlation with Tiaml. Tiaml and XAGE-lb of the correlation coefficient r=0.411, bilateral Pearson test P= 0.009 (P<0.01), statistically significant, Tiaml and XAGE-lb shows a high degree of correlation. KAI1 and XAGE-1 correlation coefficient r=-0.590, bilateral Pearson test P=0.026 (P<0.05), statistically significant, KAI1 and XAGE-lb shows negative correlation.Conclusions(1), parotid gland, compared with the control, ACC samples, low expression of KAI1, Tiaml and XAGE-1b overexpression.(2) ACC clinical stage in patients with the later and larger tumor size, KAI1 expression in the lower, Tiaml and XAGE-1b higher expression; KAI1, Tiam1, XAGE-1b expression and gender, age, and whether there is lymph node metastasis and significance.(3) KAI1 in the ACC samples were highly correlated with Tiaml, Tiam1 XAGE-1b with a high positive correlation, KAI1 and XAGE-1b high negative correlation.