Analysis Of Ovary Serous Cystadenocarcinoma And Borderline Cystadenoma Using Differential-display Proteomics

[Objective] Ovarian cancer is the leading cause of death from gynecologic malignancy. The diagnosis rate for early- stage disease is low,and prognostic is poor.This study used differential-display proteomics technology to detect the tumor marker of ovarian cancer .[Method] Using two dimensional liquid chromatography MS/MS (2-D LC-MS|MS) technology, we detected six ovary serous cystadenocarcinoma tissue samples(CA set) and six ovary borderline serous cystadenoma tissue samples(IN set),and obtain a protein expression reference of ovarian cancer, can potentially screen the specific ovarian cancer markers.[Rwsults] There are 96 differential expression protein identifications screened between CA set and IN set. 83 differential proteins are overexpression, while 13 differential proteins are down-expression. These differential proteins mainly include cytoskeletal protein,molecular chaperones,extracellular matrix protein,the proteins contributed to signal transduction,cell cycle regulation,transcription,translation,immunoloregulationand energy metabolism enzymes.[Conclusion]1.This study use 2-D LC-MS|MS technique detect tissue samples of ovary serous cystadenocarcinoma patients and ovary borderline serous cystadenoma patients . The method is efficient, repeatability, and provide a valuable resource in study on differential protein expression of human ovarian cancer.2. Of these differential expression protein identifications ,molecular chaperones,extracellular matrix protein , (especially glycoprotein),the proteins contributed to signal transduction,cell cycle regulation,transcription,translation play more important roles in carcinogenesis,advancement and invasion of ovarian cancer.3.Here we obtain some differential proteins that play key roles: vitronectin,spondin-1,heat-shock protein family,protein disulfide isomerase A3,protein disulfide-isomerase A3,prohibitin,clusterin,histone H2A.x,isoform 1 of periostin,fibromodulin,nucleolin,cathepsin D,decorin,nucleolin,etc. They could be potential tumor markers of ovarian cancer after further clinical verification, play important roles in revealing pathogenesis,diagnosis, novel treatment and prognosis evaluation of ovary cancer.