Construction Of Diabetic With Membranous Nephropathy Model And Pathology And Research Of Treg Cell

Backgrounds and objections:Diabetic nephropathy is an important complication of diabetes.It's also one of the common outcomes of death caused by chronic renal failure. DN progresses slowly. Most diabetic patients began to have microalbuminuria after 6 to 15 years when they were diagnosed diabetes, 10 to 15 years later they went into a period of large volume proteinuria. However, some clinical patients with diabetes progressed faster and quickly had severe proteinuria like nephrotic syndrome. Addition to the mechanism of kidney damage due to diabetic nephropathy ,whether there had some primary glomerular disease based on DN? The mechanism of DN pathogenesis is very complex and has not yet fully understood so far. Numerous studies show that the damage mechanism of DN involved in the immune pathogenesis. In recent years, the CD4~+CD25~+ regulatory T cells in the immune function has become a hotspot. It has been confirmed CD4~+CD25~+ Treg cells play important roles in tumor, autoimmune disease, infection, transplantation immunology, and other immune-related diseases. Do CD4~+CD25~+ ~+ Treg cells work in diabetic nephropathy with primary glomerular disease? This article aims to detect diabetic with membranous nephropathy in clinical manifestations, laboratory results, the characteristics of renal pathology and the number of CD4~+CD25~+Tr cells in peripheral blood, thymus, spleen. Providing new idears for the diagnoses and therapy of diabetic with membranous nephropathy .Research Methods:Streptozotocin, and bovine serum albumin were used to construct rat model of diabetic with membranous nephropathy. Observe their general status ,clinical characters and the 24-hour urine protein.Detect the renal pathological changes of the rat model. The number of CD4~+CD25~+Tr cells in peripheral blood , thymus and spleen of test group and the control group were detected by flow cytometry.Statistical analysis:The qualitative data of clinical and laboratory examination on present as the number of case, and the quantitative data present as mean±standard deviation. The count data used analysis of variance and chisquaretest, and measurement data by t-test, P<0.05 defined as a statistically significant difference.Results: 1 After injection of STZ, the body weight of diabetic nephropathy group and membranous nephropathy group had decreased, compared with the control group,the body of these groups are significant different (p <0.01). Although the weight of pure membranous nephropathy group gained, but the rate was significantly lower than the control group (p <0.05). Compared with the control group ,there are no significant differences of all the model groups'kidney weight (p> 0.05). However, except the membranous nephropathy group, the kidney weight / body weight of each model group was significantly higher than the control group (p <0.01), there are significant differences between the DM ~+ MN group and the MN group in kidney weight / body weight (p <0.01 ).2 diabetic nephropathy, membranous nephropathy, diabetic with membranous nephropathy group compared with the control group in 24-hour urine volume, with the extension of the course, 24-hour urine protein excretion gradually increased. Compared with the control group, MN increased urinary protein in the most obvious, DN ~+ MN group followed, DN group showed increasing the least. The urine protein in each model group compared with the normal control group were significantly different (p <0.01).3 Membranous nephropathy group's CD4 ~+ CD25 ~+ Tr cells in CD4 ~+ lymphocyte percentage was significantly decreased than the control group in peripheral blood, (P <0.05); diabetic nephropathy rats'CD4 ~+ CD25 ~+ Tr cells in CD4 ~+ lymphocyte percentage was significantly lower than the control group in peripheral blood ( P <0.05); And DN ~+ MN group's peripheral blood CD4 ~+ CD25 ~+ Tr cells compared with normal control group, the percentage is also reduced, (P <0.01).4 CD4 ~+ CD25 ~+ Tr cells in CD4 ~+ lymphocyte percentage of membranous nephropathy group was significantly higher than the normal control group in thymus (P <0.01), other groups of CD4 ~+ CD25 ~+ Tr cells in the percentage of CD4 ~+ lymphocytes showed no significant difference (P> 0.05).5 In the model rats'spleen, CD4 ~+ CD25 ~+ Tr cells in the percentage of CD4 ~+ lymphocytes shows no significant differences among these groups(P> 0.05).6 renal pathological changes: light microscopy: magnification of 400 times when, DN ~+ IMN group showed glomerular expansion, increased of glomerular mesangial cell proliferation, increased of mesangial matrix, glomerular basement membrane became thickening, stiff. Granular degeneration of tubular epithelial cells.Conclusions:1 using STZ-induced diabetic model, and built on its model of membranous nephropathy, diabetic patients can be successfully constructed into diabetic with membranous nephropathy. In general ,the model can successful state of various organs'changes of diabetic nephropathy with membranous nephropathy change.2 diabetic with membranous nephropathy in rats'pathological changesshows of both diabetes mellitus nephropathy and membranous nephropathy typical pathological changes.3 CD4~+ CD25~+ Tr cells in membranous nephropathy, diabetes mellitus and diabetic nephropathy with membranous nephropathy reduced in the proportion of CD4 ~+ lymphocytes in peripheral blood, and there is little change in the rats'thymus and spleen.