| Objective To investigate the effect of high glucose on Raman spectra of sigle living rat pancreaticβ-cells.Methods Islets were isolated from Sprague-Dawley (SD) rats. Single ratβ-cells were measured by Raman spectroscopy in 2.8 mmol/l glucose culture medium as a basal condition. After stimulation with high glucose (20 mmol/l), the same cells were measured continuously. Each cell was monitored by Raman spectroscopy over a total time span of 25 minutes, in 5 minutes intervals. The control cells were maintain 2.8 mmol/l glucose culture medium all the time.Results It was found that some significant spectral changes induced by glucose were taking place during the stimulation time course. The most noticeable changes were the increase of spectral intensity at the 1002 cm-1, 1085 cm-1, 1445 cm-1 and 1655 cm-1 peaks (p<0.05), mainly corresponding to protein and lipid.Conclusion The platform of the study on pancreaticβ-cells has been set up by the researcher. Our finding indicated that the meaningful spectral changes in pancreaticβ-cells could be detected by Raman spectroscopy. We used Raman spectroscopy for real time monitoring of acute effect of high glucose on single rat pancreaticβ-cells. During the high glucose stimulation, significant peak intensity increases were observed in the single rat pancreaticβ-cells. The high glucose stimulation was a key factor causing these positive Raman changes. To explain these phenomena, we speculate that proinsulin and lipid biosynthesis induced by high glucose may play a role.
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