Effects Of Two Methods Of Sufentanil Analgesia On Humoral Immune Function Of Consicious Rabbits

Objective:we use the formalin pathological pain model in rabbits to study the influence of two methods of sufentanil analgesia on the levels of rabbit immunoglobulin.Methods:Eighteen healthy rabbits were randomly divided into three groups (n=6 in each), namely intravenous analgesia group (group A), epidural analgesia group (group B) and control group (group C). The 5% formaldelyde solution 0.5ml was injected into the hind paw of rabbits in each group to set up formalin pathological pain model. Rabbits in group A and B were respectively administered with sufentanil 0.66μg·kg-1 intravenously and 0.92μg·kg-1 epidurally for analgesia, rabbits in the group C were injected with saline 0.5 mL intravenously as control. The spontaneous nociceptive behavior was measured by counting to assess the pain of animals, pain response was recorded with pain grade and two phase cumulative pain grade (the first phase is from 0 to 10 minutes,and the second phase is from 10 to 45 minutes.) for a period of 45 minutes. We draw-off ear edge's venous blood before the experiment (To),1 h after analgesia (T1),24 h after analgesia (T2),48 h after analgesia (T3),72 h after analgesia (T4) respectively to determine the levels of IgG, IgA and IgM by ELESA.Results:Sufentanil analgesia can reduce the pain of rabbits effectively, the pain score of group A and group B decreased steadily, while the pain of rabbits in group C last longer than that of group A and group B. The pain responses in both phase were inhibited significantly in group A and group B compared with that of group C (P<0.05). There was no significant difference in the pain responses of Group A and Group B during the two phase. There were no significant difference in the levels of IgG, IgA and IgM among three groups before experiment. The levels of immunoglobulin in three groups showed a significant decline at T1, T2 compared with T0(P<0.05), in group A and B, they rose to normal levels at the 72 h and 48 h separately. However, the control group was still remained at a low level at the 72 h.Conclusions:The pain and stress can suppress immune function, sufentanil analgesia can provide the protective effect on the stability and recovery of immune function, and the effect of epidural method is better than that of intravenous method.