Association Study Of The Children' Mentalretardation And The Forth Exon Of MeCP2 Gene

Objective:To study the mutation of MeCP2 gene'forth exon and investigate the relationship between the forth exon and the children mental retardation(MR) phenotype.Methods:Chose 30 children of MR and 30 normal children and then extracted their DNA directly from white blood cells. Then amplified the number of the MeCP2 gene by the technology of polymerase chain reaction(PCR).PCR products were separated and analyzed to find out whether there was any relationship between the forth exon of MeCP2 gene mutations and the children MR phenotype.Resuslt:No mutation of the forth exon of MeCP2 gene was found in the30 children of MR and 30 normal children.Conclusion:The forth exon of MeCP2 gene maybe not the mainly mechanism.