The Expression And Significance Of Decorin In Scars Tissue And Fibroblasts

【Objective】Measure the expression of Decorin on scar tissues and fibroblast to depth explore and reveal the role of Decorin and mechanism for the prevention and treatment of clinical and pathological on scar therapy as the theoretical basis at the molecular level.【Methods】(1) Hypertrophic scar fibroblasts were cultured in vitro,using light microscopy and electron microscopy to observe the fibroblasts' morphology, activity,apoptosis.Using the real-time quantitative PCR to detecte and analyze the expression of mRNA of Decorin and TGF-β1 in fibroblasts.(2) Scar tissue were fixed,embedded,sliced,using immunohistochemical detect the expression of the Decorin and TGF-β1 in scar tissue.By analyzing the experimental observation of above and we would study scar fibroblasts behavior and pathological,and further more to discuss the pathological role of decorin in the develoPment of scar formation.【Results】(1)Light microscopy showed:Scar fibroblasts adherent growth,spindle or polygonal,showing palisade arrangement.Compared with normal skin fibroblasts,scar fibroblasts are irregular,larger cells and arranged in disorder.(2)Electron microscopy shows:Compared with normal skin control group, with a large number of mitochondria,rough endoplasmic reticulum was reduced and see more rough endoplasmic reticulum swelling,expansion.Microtubule and microfilament in cytoplasm increased and euchromatin-rich in nucleus.Above show that the function of its protein synthesis is very active.(3)The result of real-time quantitative PCR showed that:Compared with normal skin,the expression of Decorin levels were significantly reduced in scar fibroblasts.On the other hand,the expression of TGF-β1 levels were significantly increased in scar fibroblasts and was statistically significant.(4)Immunohistochemistry on analysis showed that:Decorin was mainly expressed in normal skin dermis and collagen extracellular matrix in the fiber surface.Compared with normal skin control group,Decorin expression in scar tissue decreased;TGF-β1 expression in the scar tissue was significantly higher than in normal skin and statistically significant(P＜0.05).【Conclusions】Compared with normal skin,Decorin levels in scar tissue and fibroblast decreased significantly.Decreasing of Decorin in early wound healing prompted activity of TGF-β,which caused a large number of fibroblast proliferation,migration and excessive collagen synthesis.This may be an important factor in scar formation.