| Objectives:To investigate the pathogenesis of premature rupture of membrane (PROM)and of subclinical chorioamnionitis by detecting the level of Nuclear Transcription Factor-κBp65 (NF-κBp65) and interleukin-18 (IL-18) in maternal blood with premature rupture of membrane and subclinical chorioamnionitis.To discuss the relevance between the two factors, then to estimate their clinieal value in predieting subelinical chorioamnionitis.Methods:pregnant women of preterm premature rupture the membrane,premature rupture the membrane of term and normal pregnant women in term with intact membranes, every 25 cases were studied.The concentration of NF-κBp65 and IL-18 in maternal blood were determined by solid phase sandwi-ch enzyme linked immuno-sorbent assay(ELISA).And subclinical chorioamnionitis or not subclinical chorioamnionitis were diagnosed by HE among fifty puerperants. They were divided into two groups. The level of NF-κBp65 and IL-18 were analysed between the two groups. These experimental data were analized by the software SPSS 16.0.Results:(1) The level of NF-κBp65 in maternal blood pPROM group (2127.5±380.84)ng/ml and tPROM group (1732.54±441.81)ng/ml are significantly higher than the nomal group(1432.96±371.40)ng/ml,each group has statistically deviation(P＜0.05,P＜0.05),and the level of pPROM group is significantly higher than tPROM group(P＜0.05).(2) The level of IL-18 in maternal blood of pPROM group (51.16±6.52)ng/ml and tPROM group(47.92±5.67)ng/ml are significantly higher than the nomal group(42.20±6.42)ng/ml, each group has statistically deviation(P＜0.05,P＜0.05), and the level of pPROM group is significantly higher than tPROM group(P＜0.05).(3)The level of NF-κBp65 in maternal blood of subclinical chorioamnionitis group is (2176.78±428.86)ng/ml, The level of NF-κBp65 in maternal blood of Non-chorioamnionitis group is (1664.72±311.14)ng/ml, the level of subclinical chorioamnionitis group is significantly higher than Non-chorioamnionitis group, the two groups have statistically deviation (p＜0.05).(4)The level of IL-18in maternal blood of subclinical chorioamnionitis group is (55.32±5.63)ng/ml, The level of IL-18 in maternal blood of Non-chorioamnionitis group is (46.29±5.10)ng/ml, the level of subclinical chorioamnionitis group is significantly higher than Non-chorioamnionitis group, the two groups have statistically deviation (p＜0.05).(5)The level of NF-κBp65 and IL-18 inmaternal blood in premature rupture of membranes group is positive correlation (r=0.840,p＜0.05), but in the normal group is on correlation (r=0.382,P=0.059＞0.05); between subclinical chorioamnionitis group and Non-chorioamnionitis group is positive correlation (r=0.95,P＜0.05;r= 0.97, P＜0.05).Conclution:1,The higher level of NF-κBp65 and IL-18 in maternal blood of premature rupture the membrane may lead to premature rupture of membrane.2,The highest level of NF-κBp65 and IL-18 in maternal blood of pPROM group with subclinical chorioamnionitis, the highest level of these are correlated with infection expect PROM.3,The higher level of NF-κBp65 and IL-18 in maternal blood of subclinical chorioamnionitis group may lead to subclinical chorioamnionitis.4,The higher level of NF-κBp65 and IL-18 in maternal blood of PROM and subclinical chorioamnionitis,NF-κBp65 and IL-18 can interact with each other leading to PROM and subclinical chorioamnionitis,and identify the chorioamnionitis as valuable clinical index. |
