Effects Of Celecoxib On The Expression Of NF-κB And COX-2 In Diabetic Rats With Focal Cerebral Ischemia-reperfusion

Objective To investigate the effects of celecoxib on the expression of NF-Κb and COX-2 in diabetic rats with focal cerebral ischemia reperfusion model. Methods Diabetes model was made by intraperitoneal injection of streptozotocin (STZ) and cerebral ischemia reperfusion (IR) model was developed by longe suture occluded method in middle cerebral artery of diabetic rats. The experiment rats were divided into sham operation group(Sgroup), cerebral ischemia - reperfusion in normal saline group (NS group), celecoxib low dose group (LCIR group, 15mg / kg) and high dose group (HCIR group, 25mg/kg).30 minters following ischemia,rats were respectively given normal saline or two doses of celecoxib administered solution. 6h,12h,24h,48h after reperfusion, the rats were decapitated and the brains were collected(5 rats in the sham-operated group, and 5 rats in each of the remaining groups at each time point). The dynamic expression of NF-κB andCOX-2 in the ipsilateral cortex was detected with immunohistochemistry. The neurological deficit scores were done before each group rats killed. Results At the time point of 24h and 48h,the neurological deficit scores is not significant difference between theLCIR group and HCIR group(P>0.05)., but these two groups compare with the NS group,the different is significent(P <0.05). S group rats brain shows mild positive expression of NF-κB and COX-2. In the cortex of the rats of NS, LCIR and HCIR groups, expression of positive cells mainly surrounding the ischemic neurons, and the numbers of positive cells were more than that of S group (P <0.05).The rate of positive staining in LCIR group and HCIR group was highly reduced than that of NS group (P <0.05),while no significant difference was detected between LCIR group and HCIR group (P> 0.05). In each IR group, at 6h,12h,24h,and 48h after reperfusio(the four sub-groups), the percentage of positive labeled cells is significant different (P <0.05). The expression of COX-2 and NF-κB was obvious relevant to each other at the same time point in Ischemic brain tissue. Conclusions Celecoxib probably plays an important role in cerebral protection by inhibiting the expression of COX-2 and NF-κB in diabetic rats with cerebral ischemia-reperfusion.