| Evodia Rutaecarpa, a tradition Chinese drug, is one species of the Evodia genus of Rutaceae family, and the officinal part is its mature fruit. It has many functinal value such as analgesic, antiemetic, astringent, hypotensive activites and so on. Extraction, separation, purification, structrue and function of the alkaloids from evodia rutaecarpa were studied in this paper.1 The optimization extraction condition of the alkaloids from evodia rutaecarpaEthanol was selected as the optimum extraction solvent from three kinds of solvents: ethanol, methanol, ethyl acetate. Soxhlet was selected as the optimum efficient methods from 4 kinds of extracting methods of the alkaloids from evodia rutaecarpa: the cold soaking, ultrasonic extraction, heat reflux, Soxhlet extraction. By comparing the extraction efficient of extracting times, one extraction time was enough.Taking evodiamine and rutaecarpine as index, materials mesh, ethanol concentration, solvent multiple and extraction time were selected as the impact factors, through the response surface analysis of experimental results, the largest impact factor on the extraction rate was ethanol concentration, followed by materials mesh, extraction time and solvent multiple. The optimum extraction condition of the alkaloids from evodia rutaecarpa was: raw matetials 40 mesh, extracting time 3.0h, the solvent multiple was 31, ethanol concentration was 72%, the yield of evodiamine was 8.90mg/g.2 The separation and purification of the alkaloids from evodia rutaecarpaThrough static adsorption efficient study of evodiamine and rutaecarpine by four kinds of macroporous resin, HPD450 was selected as the adsorption resin. Ethanol was selected as the desorption solvent. Through the dynamic-eluting single factor experiment of macroporous resin HPD450, the optimum dynamic elution condition was: sample volume was 2.4mg per gram resin, adsorption rate 1mL/min, pH 9.5, 5BV 70% ethanol eluting, desorption rate was 1mL/min. By comparing the efficient of three kinds of cation exchange resin, D113 was selected to be the the adsorption resin. The efficient of cation exchange resin was better than macroporous resin by dynamic elution experiment. The sample separated by D113 was purified by Sephadex LH-20, not only evodiamine and rutaecarpine monomer, but also a mixed composition was obtained. Through the crystal and thin layer chromatagraphy, the components was further studied.3 The structrue of the alkaloids from evodia rutaecarpaThe identified peaks and characteristic group was found by using of UV and infrared spectra analysis of the alkaloids from evodia rutaecarpa. The suitable mobile phase and equipment condition for analysis single and mixed standard sample was selected with three kinds of HPLC tested. The purified extraction rate of evodiamine and rutaecarpine respectively was 95.00 mg/g and 86.00 mg/g. The preliminary purified sample was identified as five kinds of quinolone alkaloids by LC-MS analysis, four kinds of which have been reported in the literature, the other was needed to be further examined by NMR and so on.4 The antioxidant activity of the alkaloids from evodia rutaecarpaChemical model system experiments showed that the deoxidize capacity of alkaloids from evodia rutaecarpa was lower than ascorbic acid. The deoxidize ability was a positive correlation with the concentration from 0.005 mg/ mL to 0.2mg/mL. Under the equal concentration, the capacity eliminating hydroxyl radical of the alkaloids from evodia rutaecarpa was higher than that of ascorbic acid, the eliminating rate was 46% with 0.2mg/mL. the alkaloids from evodia rutaecarpa showed high capacity of eliminating superoxide anion and 2,2-diphenyl-1-picrylhydryzyl (DPPH) radicals, and the eliminating rate was about 80% with 0.05 mg/mL, while above it the eliminating rate remained almost unchanged. Under the same concentration, the capacity of eliminating superoxide anion and DPPH radicals of the alkaloids from evodia rutaecarpa was slightly higher than that of ascorbic acid. |
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