| Systematical study on the chemical constituents of traditional Chinese herbs is one of important routes to discover bioactive compounds. There are about 60 species belong to the genus Dracocephalum, family Labiatae. These plants distribute in alpine and semidry regions mainly in temperate Asia, with a few in Europe, one in North America. There are about 32 species,7 variations in China. Several species belong to the genus Dracocephalum are used as folk medicine for the treatment of tracheitis and cardiovascular disease in Xinjiang antonomous region. Dracocephalum moldavica can be applied for the protection of cardiac ischemia. This dissertation mainly focused on the chemical dtudies of the herbal plant. Bioactivities were investigated for some flavonoids. Determinations of tiliroside and agastachoside in D. moldavica were achieved by HPLC.Dracocephalum moldavica L, habitually used herb by Weiwuer and Meng nationality, mainly distributed in Heilongjiang, Jilin, Liaoning, Neimenggu, Gansu, Shanxi, Qinghai and Xinjiang in China. It was an annual herb and was cultivated in southern Xinjiang autonomous region. The aerial parts of this herb were employed in smoothing the nerves, relieveing a cough and protecting the heart. By MCI gel chromarography, silica gel chromatography and Sephadex LH-20 chromatography, thirty-one compounds were isolated from the ethanol extract of this plant. Based on spectroscopy method, all the compounds were elucidated as:ursolic acid (1), olieanic acid (2), uvaol (3), betulin (4), betulic acid (5), betulic-28-ethyl ester (6),3p,24-dihydroxyurs-12-en-28-oic acid (7), apigenin (8), apigenin-7-O-glucopyranoside (9), apigenin-7-O-galactopyranoside (10), luteolin (11), luteolin-7-O-glucoside (12), acacetin (13), acacetin-6"-glucuronide (14), gardenin B (15), scrophulein (16), salvigenin(17), isorhamnetin (18), tilianin (19), agastachoside (20), acacetin-7-O-glucoside-6"-O-malonyl ester (21), takakin-8-O-β-D-glucopyranoside (22), kaempferol (23), kaempferol-3-O-βD-(6"-O-p-coumaroyl)-galactopyranoside (24), kaempferol-3-O-β-D-(6"-O-p-coumaroyl)-glucopyranoside (25),2"-p-coumary lastra-galin(26), methyl rosmarinate (27), ethyl rosmarinate (28), stigmasterol (29), P-sitosterol (30), daucosterol (31). Compounds 10,27and 28 were isolated from the genus of Dracocepahlum for the first time,4,5,6 and 9 were isolated from this specie for the first time.As a transcription factor, Nrf2 regulated the expression of phaseⅡand cellular detoxification enzymes, which could protect cells from radical and external toxic. Studies had shown that induction of Nrf2 expression was helpful for the treatment of a number of chronic diseases such as cardiovascular disease, diabetes. Some of the compounds we obtained were screened the Nrf2 inducing activity using a stable cell line which could express luciferase. Results showed that most flavonoids tested could induce of the expression of luciferase and could be potential Nrf2 activitors. This effect might be related to the heart and brain protection of D. moldavica.Using HPLC method, the contents of tilianin and agastachoside in D. moldavica were determined. The flaconoids contents in different seasons of D. moldavica were compared. It indicated that the HPLC method was suitable for the quality evaluation of the herb.
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