| OBJECTIVE:To approach effect of ADM aiming osteanagenesis in immediately tooth implantation. To observe the different effects on the quantity of new generated bone betweenβ-TCP and CHA under the role of ADM Biomembrane in alveolar augmentation operation of immediately implant in a dog model.METHOD:8 healthy male mongrels and 48 implants were used to build the animal model. Divided into 6 groups:Group CHA+ADM (A)/Groupβ-TCP+ADM (B) /Group CHA (C)/Groupβ-TCP (D)/Group ADM (E)/Control group (F). Plant 48 Branmark implants into the mandible immediately after pulling out 48 premolar teeth on four inferior maxilla form sides of adult male Beagle dog altogether.48 implants (φ=4.1mm,L=10mm) were embedded which the depth in mandible was 10 mm, and bone defects of 3mm×4mm×5mm were prepared in the cheek side of alveolar bone. Bone regeneration was observed by X-ray and histological observation. Measure the distance of bone defect bottom between implant cervical margin after reparation,bone defect zonal's bone density, implant sclerous tissues slice TB dyeing,to observe new bone formation. Correlated data does statistics analysis.RESULT:None implant failed, defect decayed area mucous membranes accrete well, and no soft tissue splits. Group A:General observation of samples:In first month, the involucrum became thin and the artificial bone materials began to absorb in the defect; while in fourth month, most of the involucrum was absorbed, the new bone tissue filled in the defect and combined well with the implant. X-ray observation:In first month, the density of bone defects is inhomogenous, shows the absorption images of some artificial bone materials; in the fourth month, the density of bone defects was uniform and continuous, close to the images of surrounding tissues. Histological observation:In first month, the new bone grew along the edge of the bony wall toward the center of the defect, the new bone trabeculae connected to each other and show meshy distributing, with connective tissue formed near the neck of teeth; in the fourth month, the bone defect was repaired with lamellar bone, Harvard's system can be seen, inside the trabecular bone is a large amount of bone lacunas, surrounded by a small amount of osteoblasts, the new bone connected with implant formed solid osteointegration. The generation rate of involucrum in 1 month and 4 months was 23.00%±0.82%,58.00%±1.83%. The distance from neck margin of the implant to the defect level was 1.05±0.13mm,1.09±0.10mm; Group B:General observation of samples:In first month, the artificial bone materials began to absorb and with bone-like tissue formation in the defect; in the fourth month, the new bone tissue filled in the defect and combined well with the implant. X-ray observation:In first month, the density of bone defects was inhomogenous, shows a large number of granular high-density images; in the fourth month, the density of bone defects is uniform and continuous, close to the images of surrounding tissues, still shows some high-density particle images. Histological observation:In first month, the new bone trabeculae can be seen in the bone defect, with connective tissue formed near the neck of teeth; in the fourth month, the bone defect was repaired with lamellar bone, inside the trabecular bone was large amount of bone lacunas, the new bone connected with implant formed solid osteointegration. The generation rate of involucrum in 1 month and 4 months was23.75%±0.96%,57.75%±1.89%. The distance from neck margin of the implant to the defect level was 1.09±0.10mm,1.13±0.10mm; Group C:General observation of samples:In first month, some soft tissue growed in the bone defect, part of the artificial bone materials was absorbed and with few bone-like tissue formation; in the fourth month, the bone defect was filled with some soft tissue and new bone-like tissue, with artificial bone materials absorbed obviously. X-ray observation:In first month, the density of bone defects is inhomogenous, shows the absorption images of some artificial bone materials; in the fourth month, the density of bone defects is uniform and continuous, but lower than the density of the surrounding tissues. Histological observation:In first month, the new bone was formed and the new bone trabeculae connected to each other reticular, with connective tissue formed near the neck teeth; in the fourth month, the bone defect was repaired with lamellar bone. The generation rate of involucrum in 1 month and 4 months was 21.00%±0.82%,51.75%±1.89%. The distance from neck margin of the implant to the defect level was 2.88±0.10mm,2.93±0.17mm; Group D:General observation of samples:In first month, some soft tissue growed in the bone defect, granular bone pate can be seen, a small amount of new bone-like tissue formation; in the fourth month, the bone defect was filled with some soft tissue and new bone-like tissue, with artificial bone materials absorbed obviously. X-ray observation:In first month, the density of bone defects is inhomogenous, shows granular high-density images; in the fourth month, the density of bone defects is uniform and continuous, but lower than the density of the surrounding tissues, still shows few granular high-density images. Histological observation:In first month, the new bone was formed and soft tissue formed near the neck of teeth; in the fourth month, the bone defect was repaired with lamellar bone. The generation rate of involucrum in 1 month and 4 months was 19.75%±0.96%,51.00%±1.83. The distance from neck margin of the implant to the defect level was 2.85±0.13mm,2.90±0.14mm; Group E:General observation of samples:In first month, the involucrum became thin and the new bone formed in the defect; in the fourth month, the involucrum was absorbed, a large number of new bone filled the defect and connected closely with the imlplant. X-ray observation:In first month, the defect was clear; in the fourth month, the bone density was increased in defect, but still lower than the density of the surrounding tissues. Histological observation:In first month, the new bone grow along the edge of the bony wall toward the center of the defect, with connective tissue formed near the neck of teeth; but there is no connective tissue in the most area of the bone defect; in the fourth month, the bone defect was repaired with a liitle lamellar bone. The generation rate of involucrum in 1 month and 4 months was 18.00%±0.82%,48.50%±1.91%. The distance from neck margin of the implant to the defect level was 2.95±0.13mm, 3.00±0.22mm; Group F:General observation of samples:In first month, the mucosal of the defect slightly sunk, fibrous connective tissue can be seen; in the fourth month, the defect was gradually filled with new bone tissue, surrounded most of the implant, but some fibrous connective tissue still can be seen near the top part of alveolar. X-ray observation:In first month, the defect was clear; in the fourth month, the bone density was increased in defect, but still lower than the density of the surrounding tissues. The generation rate of involucrum in 1 month and 4 months was 4.25%±0.96%, 16.50%±1.91%.The distance from neck margin of the implant to the defect level was 4.38±0.18mm,3.75±0.13mm. The statistical analysis of new bone formation rate in defect is:In first/fourth month, there is no significant difference among A and B, C and E, C and D, while there are significant differences statistically in comparison between any two groups. Compared with the first month, the new bone formation rate of the fourth month is higher, with statistical significance. The statistical analysis of the distance from neck margin of the implant to the defect level is:In first/fourth month, there is no significant difference among A and B, C and D,C and E, D and E, while There are significant differences statistically in comparison between any two groups. The paired t test of each group in the first and fourth month, ABCDE group P>0.05, with no significant difference, F group P<0.05, with statistically significant.CONCLUSION:1. Using of GBR technology in immediately dental implant can obviously increase the speed and the quantity of new generated bone.2. ADM Biomembrane can aim osteanagenesis. ADM Biomembrane mixed with artificial bone materials are superior to that of ADM Biomembrane orβ-TCP or CHA.3. ADM Biomembrane and B-TCP both can aim osteanagenesis,they both can be used to repair bone defect in immediately dental implant. |
PDF Full Text Request