Experimental Study On Apoptosis Of Two Cancer Cells Induced By Analogues Of Curcumin

Curcumin is the active ingredient extracted from turmeric, with the Chemical formula of C21H20O6 and a molecular weight of 368.39.Curcumin, with anti-tumor, anti-inflammatory and cardiovascular protection and other pharmacological effects, was instability in vivo.A number of single-carbonyl analogues of curcumin with stable structure were designed and synthesized in the previous experiments.Their pharmacological effects in vitro was detected in this paper in order to provide clues for the antitumor effect of curcumin analogues.The inhibitory activity of 17 single-carbonyl analogues of curcumin to H460 and MCF-7 was studied by MTT.The results obtained by MTT was that the inhibitory effect of A75,B67 to H460 cells and B67,C67,A69 to MCF-7 cells was significant.One drug had different influence on different cell line.Observed by optical microscope,fluorescence microscope,laser scanning confocal microscope, single-carbonyl analogues of curcumin B67,C67,A69 could induce apoptosis of MCF-7 cells.The effect of B67,C67,A69 restraining MCF-7 proliferating associated with the efficacy of their inducing cell apoptosis.The denser the drug is,the higher the cell apoptosis rate there is.Observed by optical microscopy, fluorescence microscopy, flow cytometry, single-carbonyl analogues of curcumin A75,B67 could induce apoptosis of H460 cells. Further more, apoptosis was confirmed related to bcl-2 gene by Western blot.