Expression Of Bcl-2 And Collagen Ⅰ At Varied Levels After Repair Of Bile Duct Injury

Objective To explore the effect of Bcl-2 and collagenⅠon repair of bile duct injury of dog at different level.Methods 30 Beagle dogs were randomly divided into control group,BDI group, BDI-repaired group.The animal model was built according to Davidoff 'typical' model of bile duct injury related laparoscopic cholecystectomy.On the fifteenth day after model building,animals were killed,histopathology examination of bile duct was performed.All samples were observe with HE staining and detected expression of Bcl-2 and collagenⅠon bile ducts with MaxVision Method.Then, analysis system measured all specimens for expression rate of Bcl-2 positive celles in fibroblasts and integrated optical density(IOD) of Bcl-2 protein andcollagenⅠ.Results Light microscopy showed that mucous membrane exuviated,arrangement of collagen fibers was tangle,new vessels increasing and vessel wall thicking in BDI -repaired group.The nearer to low level,IOD of Bcl-2 in BDI-repaired group increased more obviously;median or low level of BDI-repaired group was significantly higher than high level(P＜0.05),but between the low and median level did not reach statistical significance(P＞0.05);IOD of Bcl-2 at three levels of BDI -repaired group was significantly higher than the corresponding level of control group or BDI group(P＜0.05);at the high level or median level,the corresponding level of between BDI group and control group did not reach statistical significance(P＞0.05);but at low level,between BDI group and control group reached statistical significance(P＜0.05).The nearer to low level,expression rate of Bcl-2 positive celles in fibroblasts(R-Bcl-2-FB) in BDI-repaired group increased more obviously;median level or low level of BDI-repaired group was significantly higher than high level(P＜0.05),but between the low and median did not reach statistical significance(P＞0.05);R-Bcl-2-FB at the low level of BDI group is higher than high level(P＜0.05),between median 1 and low level or high level did not reach statistical significance(P＞0.05);R-Bcl-2-FB at three levels of BDI-repaired group was significantly higher than the corresponding level of control group or BDI group(P＜0.05);at the high level or median level,the corresponding level of between BDI group and control group did not reach statistical significance(P＞0.05);but at low level,between BDI group and control group reached statistical significance(P＜0.05).There was remarkably difference among IOD of collagenⅠat the three levels in BDI-repair group.(P＜0.05);in BDI group,high level is remarkably lower than median level or low level(P＜0.05);between the low level and median level did not reach statistical significance(P＞0.05);IOD of collagenⅠat three levels of BDI repair group is significantly higher than the corresponding level of control group or BDI group(P＜0.05);at the high level,between BDI group and control group did not reach statistical significance(P＞0.05);while at the medium or low,the corresponding level of between BDI group and control group reached statistical significance(P＜0.05).Conlusion The quantity of expression of Bcl-2,expression rate of Bcl-2 positive celles in fibroblasts and collagenⅠin BDI-repair group increased and was different on different lever,which was relatively more on close to duodenum(low lever) than on close to hepatic porta(high lever).In the same background of BDI-repair group, the expression of Bcl-2,FB-Bcl-2 and collagenⅠ,the closer the level to low lever,the higher at the level.Because of the direct correlation between postoperative scar accrementition and restenosis of bile duct and Bcl-2 or collagenⅠ,the incidence rate of restenosis at high lever of bile duct jejunum anastomosis is lower than that at low lever.