| Objective:Epidermal Growth Factor Receptor, a member of the typeâ… growth factor receptor family, is closely related with many types of cancers. However, its related signal transduction pathways and proteins are still incompletely known. The aim of the current study was to verify that EGFR regulates CyclinE expression in Non-small-cell lung carcinoma (NSCLC). The results would help us to further understand EGFR and its related signal transduction pathway in NSCLC, and provide rationale and experimental proof for targeted therapy.Methods:â—Westernblotting and Real-time PCR were used for observing the EGFR expression in three NSCLC cell lines.â—RNA interference technology was used for inhibiting EGFR in EGFR highly-expressed cell lines. Western blotting was used for detecting EGFR and Cyclin E expression.Results:â—In the condition that the sample contents of proteins and RNA were equal, western blotting detected that EGFR expressed in SPC-A-1 and SK-MES-1, while not in NCI-H661; Real-time PCR revealed that EGFR expression in SPC-A-1 and SK-MES-1 was high, while in NCI-H661 was low.â—The expression of EGFR and CyclinE in SPC-A-1 and SK-MES-1 decreased after RNA interference.Conlusions:â—EGFR expresses in pulmonary adenocarcinoma and squamous cell carcinoma cell lines, while not in large cell lung cancer.â—EGFR regulates CyclinE expression in pulmonary adenocarcinoma and squamous cell carcinoma cell lines. |
