The Expression And Significance Of CyclinE, Ki-67, EGFR And Bcl-2 In Seborrheic Keratosis

Seborrheic keratosis (seborrheic keratosis SK) is a common benign tumor of the skin, prone to the exposure of the old, mostly brown or dark brown stain film or plaque, affect significantly on the appearance of the skin condition. It is easy to cause friction and inflammation after stimulation and cause crusting, hyper- keratosis or hyperplasia, which speed up and increase opportunities for misdiagnosis. Individual cases can be transformed or combined with other malignant tumors. In order to meet the needs of increasing attendance rate year after year, this article on the part of the mechanism of pathogenesis seborrheic keratosis, provide basis for improving treatment。Domestic and foreign scholars believe that the nature of seborrheic keratosis is a monoclonal tumor, characterized by basaloid cell proliferation, accompanied by varying degrees of squamous cell differentiation. Seborrheic keratosis tumor mitosis sometimes obvious, the obvious apoptosis can occur in the squamous differentiation of the region。In recent years, there is a lot studies about the pathogenesis of seborrheic keratosis around the keratinocyte proliferation, differentiation apoptosis and the regulation of diagonalization observation and tests, which provides a large number of reference with seborrheic keratosis of the study.In order to further observe the proliferation and apoptosis in the mechanism of seborrheic keratosis, we chose four kinds of seborrheic keratosis may be related to accelerated proliferation, apoptosis inhibitory factor and observe its expression to analyze the relationship between its expression in order to provide basis for the pathogenesis of seborrheic keratosis .In this study, using SP immunohistochemical staining method, positive cells counted under a microscope and the use of Image-Pro plus 6.0 image acquisition software analysis system and integrated optical density (IOD) values ,we observed CyclinE, Ki-67, EGFR and Bcl-2 in seborrheic keratosis and normal skin tissue expression, from the perspective of cell proliferation and apoptosis we discuss the four regulatory factors in the pathogenesis of SK, so as to excess keratosis awareness and prognosis research reference. The results are as follows:1. CyclinE positive positioning in the perinuclear cytoplasm, brown-yellow granules. In normal group, it only express in the basal layer epidermis, and the positive rate was 10%; In SK group of lesions ,it expressed in basal cell hyperplasia-like areas, the positive rate was 64%. CyclinE average expression in normal epidermis points to 1.10±0.31, in SK, the average expression of points is 1.92±0.60; two groups of expression intensity data use rank sum test, uc = 3.058, P <0.05, it has significant difference between the two groups. The results showed that CyclinE expression intensity of SK tissue was significantly higher than the normal epidermis.2. Ki-67 expression in all proliferating cells in the nucleus, shown as brown- yellow granules, in the basal layer of the epidermis of 10 cases of normal skin ,the individual spines nucleus have been shaded, the positive rate is 20%; 50 cases of SK lesions more common in the basal cell layer and higher layers, the expression of full-thickness is rare, positive expression rate is 86 %; The average points of expression of Ki-67 staining in normal epidermis is 2.20±0.42, in SK group, the average expression of points is 2.70±0.46. Expression intensity of two samples with rank sum test, uc = 3.963, P <0.05, it has significant difference between the two groups ,so the result showed that the high expression of Ki-67 in the SK became apparent.3. EGFR-positive reaction product located in the cell membrane, some are also expressed in the cytoplasm, expressed as brown or brown granules. In normal skin the basal layer and part of spine expression gradually reduce upward, no expression of cuticle. The positive expression rate is 40%; In SK lesions, the expression of a wide range in the stratum corneum, granular layer and the upper spine and the basal cells, including acanthosis with hyperkeratosis or hypertrophic, or nipple tumor-like proliferation was strongly positive; The positive expression rate is 94%. EGFR staining in normal skin, the average expression of points is1.70±0.48, in SK Group, the expression of average points is 2.46±0.57. Expression intensity of the two groups of samples with rank sum test, uc = 4.207, P <0.05, it has significant difference between the two groups. The results showed that EGFR expression in the SK was stronger than that in normal skin4. Bcl-2 positive reaction located in the cytoplasm, expressed as brown or brown granules. In the normal group, it only express in the basal layer of skin , the positive expression rate is 50%; it expressed widely in the basal layer of SK lesions, weakly in the lower stratum spinosum,the positive expression rate is 68%; Bcl-2 staining in normal skin expression , the average expression of Points is 1.04±0.52,in SK, the average expression of points is1.38±0.56. Expression intensity of the two groups of samples with rank sum test, uc = 1.592, P> 0.05, it does not have significant difference between the two groups. The results showed that in the SK tissue , the expression of Bcl-2 has the trend of higher than normal skin, but no statistical significance.5. CyclinE, Ki-67, EGFR, and Bcl-2 in 50 cases of SK lesions tissues correlation analysis showed that, CyclinE and EGFR, EGFR, and Bcl-2, CyclinE and Ki-67 expression intensity in the two samples all have correlation, P <0.01;The expression showing a significant trend of more positive correlation, indicate four kinds of protein has synergy in the pathogenesis of the SK.Conclusion:①High expression of CyclinE in seborrheic keratosis-like cell hyperplasia of the basal zone indicate cell proliferation seborrheic keratosis and CyclinE over-expression。②Ki-67 was significantly higher expressed in seborrheic keratosis in addition to the stratum corneum in the outer layers of epidermal cells, suggesting that seborrheic keratosis cells a wide range of proliferation activity with the Ki-67 over-expression-related③EGFR in seborrheic keratosis expression of full-thickness tissue was significantly higher than in normal skin, indicating over-expression of EGFR and the proliferation of seborrheic keratosis excessive apoptosis-related;④Bcl-2 expression in seborrheic keratosis was not obvious, suggesting that seborrheic keratosis of Bcl-2 inhibition of apoptosis alone is not obvious;⑤CyclinE and EGFR, EGFR, and Bcl-2, the expression of CyclinE and Ki-67 in seborrheic keratosis has a significant positive trend in correlation, indicating three groups of proteins have synergies in the pathogenesis of seborrheic keratosis.