Study The Roles Of Tea Polyphenols For Periodontal Ligament Cells

Introduction:parodontosis is most of generally the diseases about oral disease,archaccumulation odontoperiosteum,on circum-tooth gingivitis and periodontal pocket emerged when parodontosis,collagen typeâ… fibers is major reconstitute which reside(in) dental capsule and major infected tissue by oral bacterium,eventually periodontal membrane space widen,sustain force of alveolodental ligament breakdown or lost, frontal merging,tooth cinch and ablate,in fine loss tooth.Severity influence patient's beautiful outlook,pronunciation,chew and digestive tract function,detriment physical and mental health,attack rate to reach 80%about.But the sup-factor about curative of cacodontia is periodontal ligament by destroy,how inhibit inflammatory factor(major is LPS) and enhancement periodontal new adherent form became the emphasis about the study.Tea polyphenols is Polyphenols materials which are extracted in green tea,are pharmacodynamic arch-active component of tea,the maximal substance is epigallocatechin-3-gallate(EGCG) in tea polyphenols contents,EGCG is one of best strong about biological effect in catching chemical compound.TP not only possess anti-oxygen,lipid per oxidation,clean up free radical,anti- catastrophe and anti- tumor generation,eta,Biologic activity and pharmacy-effect,but also possess very strong role of anti-inflammatory and anti- autoantibody.With healing many inflame disease have fairly large invest value.This experiment alternative LPS and TP became experiment medication.Objective:detect the proliferation of TP for periodontal ligamentcell(PDLC);establish periodontal inflammation model,detect the role of TP for periodontal inflammation model.Method:Essays divide vivo and vitro.TP could be prepare to difference concentration of TP aqueous solution by PBS,aetio-generation alveolodental ligament cell with cover slip and concoction architecture clump method cultivate,second filial generation cell progress cello- accredit,cum third generation cell for vitro pharmacodynamic experiment model,with LPS excite PDLC,view effect what is disparity concentration TP aqueous solution duplet periodontal ligament corpuscular propagate and LPS depressant for it and periodontal ligament repair by method MTT,with differentiation what is tea polyphenols anti-PDLC by method MTT;detect variation of type collagen I fibers protein level when TP action PDLC with method ELISA.Experimental model by erect rat SD experimental cementoperiostitis for vivo drug action with ligature wire ligate the rat's dental cervix,inject hormone and high glucose,view the variation about odontoperiosteum morphology and evaluation the curative effect about TP to cure peridentitis by histological section and HE stain.Consequence:TP have generation effect for PDLC,and it is concentration dependent (P=0.00),5ug/ml,10ug/ml,50ug/ml,100ug/ml and 200ug/ml TP water solution group with control group compare,TP water solution group can promote proliferation of PDLC.200ug/ml had better effect(P=0.00),300ug/ml could be result in cell proliferation decrease.The effect time is TP contributed the PDLC,but timeless difference(P=0.051). LPS can inhibit the growth of PDLC,it also have concentration dependent and time dependence(P=0.017),when LPS excited the PDLC 24h and 48h that 200ug/ml TP aqua can available depressant effect(P=0.040P=0.019).Consequence indicated from ALP activity determination and collagen protein typeâ… assaying,TP can elevate the differentiate effect of PDLC when cultivate PDLC with TP. And it have concentration dependent(P=0.005),200ug/ml is the best concentration, P=0.07,un-time dependence.With increase of concentration and extend of time,collagen protein typeâ… content increase,after PDLC stimulated by LPS,TP have marked depressant effect for it,TP can active suppression LPS which is demolish the collagen protein typeâ… in periodontal,and 48h better than 24h.(P=0.003,P=0.001).The curative effect of TP for essay peridentitis:2mg/ml TP aqua can inhibit periodontal demolish about peridentitis model,enhancement regenerate of periodontal ligament,it is play role of precaution and become one of adjuvanticity curative drug for peridentitis.Conclusion:TP aqua can enhance generation and vitro differential of PDLC,encourage collagen typeâ… protein level augment,the best effect of time and level is 24h,200ug/ml. it also can inhibit breakdown for PDLC by LPS,facilitate excrete of collagen typeâ… in PDLC,24h,200ug/ml TP aqua is the best effect of containment.Periodontal were ligated, Injection of hormone and high glucose is one of utility,safety,stabile method for establish parodontosis animal model.2mg/ml saturation TP have effect of management the rat SD parodontosis model by drinking water,inhibit inflamed destruction,and diminish the demolition degree,have tendency of promote repair for dental capsule and repair of periodontal ligament.