| Asthma is a chronic obstructive airway disease featuring eosinophilic airway inflammation,airway hyperesponssiveness(AHR) to bronchospasmogenic stimuli, mucus hypersecretion,airway remodeling,elevated serum levels of allergen-specific IgE and unbalanced Th1/Th2 immune responses.CD4+CD25+T cell andγδT cell participate in the allergic airway inflammation.Cytokines and chemokines play a key role in orchestrating the chronic inflammation of asthma.CXCR3 receptor and its ligands are nearly correlative with Th1 immune response.In this paper,we explore the function of CD4+CD25+T cell,γδT cell and CXCR3 by setting up an asthma mice model sensitized and challenged with house dust mite extracts Dermatophagoides farinae.In this study,pathological manifestation of the lung,cell counts and classification in BALF were studied;protein in the BALF was detected by BCA;IL-4 and IFN-γ,levels in BALF and spleen supernatants were detected by ELISA;CD4+T cell,CD8+T cell,CD4+CD25+T cell andγδT cell in the lung and spleen were detected by flow cytometry;the expression of Mig,IP-10,TGF-β,IL-10 and Foxp3 mRNA were detected by RT-PCR.In the end,we found that there was pulmonary eosinophilic inflammation in the mice sensitized and challenged with house dust mite extracts.Total cells counts, eosinophil counts and IL-4 levels in BALF and cultured splenocyte supernatants enhanced significantly while IFN-γdecreased(P＜0.01) in HDM Groups.The proportion of CD4+CD25+T cell in HDM groups increased significantly(P＜0.05) in the lung while decreased significantly(P＜0.05) in the spleen.The expression of TGF-βand IL-10 mRNA in the HDM groups were lower than the control groups (P＜0.05).The proportion ofγδT cell in HDM groups increased significantly(P＜0.05) in the lung while decreased significantly(P＜0.05) in the spleen.The changes of total cells counts,eosinophil counts,lymphocyte counts,protein in the BALF,IL-4 in the splenocyte supematants and pathological manifestation of the lung were more intensive in CXCR3-/- mice than C57BL/6 wild-type mice.Further more,the expression of Mig,IP-10 mRNA in CXCR3-/- mice lung were lower than C57BL/6 wild-type mice(P＜0.05).CD4+CD25+T cell modulates the allergic pulmonary inflammatory reaction depended on IL-10 and TGF-β.The allergic pulmonary inflammation may be suppressed by uprising IL-10 and TGF-β.γδT cell participates in the allergic pulmonary inflammation,but the immanent mechanism needs further study.As compared with C57BL/6 wild-type mice,CXCR3-/-mice were more sensitized to allergic pulmonary inflammation model.CXCR3 and its ligands(Mig,IP-10 and I-TAC) play an anti-inflammatory role during the pathogenic process in this animal model.They may be protective factors for asthma patients.Promoting the expression of CXCR3 and its ligands could be a new target for preventing and curing asthma. |
