| The stability and antioxidant activity in vitro of rosmarinic acid(RosA) was researched systematically and the antioxidant activity in vivo effect of RosA on D-galactose (D-gal) induced aging mice was conducted in this paper.The stability of RosA had a more comprehensive study to identify the factors that influence the stability for the safe use of RosA and the further development and utilization. The results indicate that illumination was a significant factor on the stability of RosA. Temperature or pH was a trifling factor on the stability of RosA.Ca2+,Mg2+ will lead to reduce the content of RosA.Salt,sugar does not affect the stability of RosA.Low concentration of sodium benzoate,potassium sorbate,ascorbic acid or oxidant was not a significant factor on the stability of RosA.Sodium pyrosulfite on the impact was not obvious.The antioxidant ability in vitro of RosA was studied.The RosA showed stronger scavenging ability on hydroxyl radical(·OH),H2O2 and·ABTS than ascorbic acid. However the RosA showed slightly lower scavenging ability on superoxide radical(·O2-), inhibiting activity on liposome peroxidation,reducing power than ascorbic acid.To investigate the antioxidant ability in vivo,we established the oxidative damage model of Balb/c mice induced by D-gal in vivo.Injected subcutenously with D-gal (100mg/kg body weight),mice in model group exhibited significant symptoms,which indicated the aging model was established successfully in this study,such as decreased serum,liver and brain SOD and CAT,enhanced MDA.Middle and low doses of RosA (100,200mg/kg) increased serum and liver SOD and CAT activity,middle and high dose of RosA(200,400mg/kg) increased the activity of antioxidative enzyme in brain.Middle dose of RosA reduce serum,liver and brain MDA in both male and female mice.These results indicated that antioxidant activity of RosA was played an important role of protection. |
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