Clinical Research Of High Risk Human Papillomavirus Infection In Breast Cancer Tissues

Background Breast cancer is one of the most common malignant tumor in women, the incidence of which is of an upward trend in recent years. It has been reported that in some big cities breast cancer has been the first of female malignant tumor. The etiology of it includes hormone, genetic factor, gene mutation, biologic agent, chemical factor and physical agent and so on. Human papillomavirus has been found to be associated with several human cancers, includes carcinoma of uterine cervix, laryngeal, urinary bladder, esophageal, anus and so on. High risk human papillomavirus has been proved to be an essential factor of some genital system malignant tumors just as uterine cervix cancer .Though there are a few relative researches, Some researches show breast cancer associate with HPV, while others got the contrary result. The infection of HPV in breast cancer tissues is uncertain and the association between HPV infection and the genesis and progression of breast cancer is still unclear.Objective To examine the infection of high risk human papillomavirus (HR-HPV) in breast cancer tissues and determine the association between HR-HPV infection and clinical and pathology characters of breast cancer. Methods The exfoliated cells were collected from 85 malignant and 20 benign breast tumors. 13 subtypes HR-HPV DNA were detected by hybrid capture 2(HC2) system (HPV-16,18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68 ) . ER, PR and Her-2 of all the breast tumors were also detected. According to the results, use the SPSS 13.0 software for statistical analysis, and the method is X test, the difference between breast cancer and benign diseases and the correlation between HR-HPV infection and the breast cancer were investigated.Results HR-HPV DNA was positive in 26 out of 85 breast cancer tumors and none of 20 benign breast diseases(P=0. 01). 69.2% breast cancer tumors are HR-HPV DNA positive before age 40 while in the patients after age 40 the HR-HPV DNA positive rate is 23.6% (P=0.027). There is no difference between the breast tumors with different clinical and pathology characters.Conclusions HR-HPV DNA infection exists in breast cancer tissues and it probably runs a roll in the onset of breast cancer, but there is no association between HR-HPV infection and clinical and pathology characters of breast cancer.