Expression Of Grp94 And TAG-72 And Their Clinicopathological Significances In Primary Hepatic Carcinoma

Objective To investigate the expressions of Glucose Regulated Protein 94 (Grp94) and Tumor Associated Glycoprotein 72 (TAG-72) in primary hepatic carcinoma (PHC), and the relationship between themselves. And to explore whether or not there is relationship betweent the expressions of Grp94 and TAG-72 and the clinicopathological characteristics of PHC.Methods The specimens from 48 patients with primary hepatic carcinoma (PHC) and 20 corresponding peritumoral hepatic tissues were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 4μm-thick sections. EnVision^TM immunohistochemistry was performed to detect the expressions of Grp94 and TAG-72 with monoclonal antibodies and scored them under high-power microscopy.Results In the 48 PHC cases, there are significantly statistical difference in the expressive positive rate and the expressive scores of Grp94 and TAG-72 between tumor and peritumoral tissues (P<0.01) .The tumor tissues with intrahepatic metastasis and tumor thrombosis have significantly higher expressive positive rate and expressive scores of Grp94 than the others without metastasis and tunor thrombsis (P<0.05 ) . But no relatioship between Grp94 and other main clinicopathological characteristics of PHC (P>0.05).Except with the descending differentiation degree (P<0.05) there is no clinicopathological characteristics of PHC correlated with the expression of TAG-72 (P>0.05).There is no evident of correlation between the expressive scores of Grp94和TAG-72 in cancer tissues of PHC (P>0.05) .Conclusion1. The significant overexpression of Grp94 in PHC may suggest that it play an impotant role in the invasive and metastatic process.2.The significant overexpression of TAG-72 may suggest that it may have something to do with the differentiation of PHC.3.There is no correlation between the expressive scores of Grp94 and TAG-72 in tumor tissues of PHC.