The Expression Of PI3K,Akt,ERK1/2 In Colorectal Carcinoma And Their Clinical Significance

OBJECTIVE: To study the expression of PI3K,Akt and ERK1/2 in colorectal carcinoma and the relationship between the expression and the clinicopathological characteristics.METHODS: To select 40 fresh specimens taken from resected colorectal carcinoma and another 40 from the tissue beside the colorectal carcinoma. Tissue sections were stained by EnVision^TM immunohistochemistry with Akt as polyclonal antibody. Tissue sections were stained by immunohistochemical S-P method with PI3KP85 as polyclonal antibody and ERK1/2 monoclonal antibody . The expression of PI3K , Akt and ERK1/2 in colorectal carcinoma and the relationship between the expression and the clinicopathological characteristics were studied with X² check,t check and the Spearman grade relevance analyse.RESULT: 1.Positive expression of PI3K and Akt in the tissue of colorectal carcinoma were 67.5% and 75.0% , which were obviously higher than those of the tissue beside the colorectal carcinoma (P <0.01). In the colorectal carcinoma,the expression of PI3K and Akt had a positive correlation (r = 0.687, p <0.01). 2,Positive expression of ERK1/2 in the colorectal carcinoma was 57.5% , which was obviously higher than that in the tissue beside the colorectal carcinoma (P <0.01). 3.The expression of PI3K , Akt and ERK1/2 in the colorectal carcinoma were not correlated with the age , tumour location , pathological type , clinical stages, lymph node metastasis and distant metastasis (p>0.05). The expression of PI3K and Akt were not correlated with that of ERK1/2 in colorectal carcinoma (P> 0.05).CONCLUSION: 1, The expression of PI3K and Akt increased obviously in the colorectal carcinoma . In the colorectal carcinoma , a positive correlation was observed between the expression of PI3K and that of Akt,but they had no correlativity with the clinicopathological characteristics. It indicated that the PI3K/Akt signal passway may be an early event of the occurrence and development of the colorectal carcinoma. The abnormal activation of PI3K/Akt signal passway played a certain role in the formation of the colorectal carcinoma.2, The expression of ERK1/2 increased obviously in the colorectal carcinoma . It showed that the expression of ERK1/2 was related to the development of the colorectal carcinoma.