Expressions Of HSP27 And IGF-1 In Brain Tissue Following Cerebral Ischemic Reperfusion In Gerbils And Neuroprotective Effects Of Them

ObjectiveTo explore the expression and the protective mechanisms of heat shock protein 27 (HSP27) and Insulin-like growth factor-1 (IGF-1) in brain tissue following cerebral ischemic reperfusion in Mongolian gerbils and whether they are correlative.Methods(1).Animal Groups: thirty healthy male Mongolian gerbils are devided into three groups:Normal Group (n=5); Sham Operation Group (n=5); Ischemia Reperfusion Group (I/R) (n=20); I/R Group are devided into four subgroups (each group n=5): 6 hours, 1 day, 3 days ,7 days after I/R;(2).Models: Making Mongolian gerbils underwent a transient 10 min bilateral carotid artery occlusion(CAO) followed by various reperfusion times (6 h, 1 d, 3d, 7d); The Sham Operation Group only give segregation but no occlusion;(3).Histological analysis of tissue injury: staining with stained withhaematoxylin and eosin haematoxylin and eosin (HE).(4).The expressions of HSP27 and IGF-1 in brain tissue were detected by using immunohistochemistry technique at the 6th hours, 1st day, 3rd and 7th day after IR;(5).Experimental results were analyzed with the statistic soft package of spss15.0.Result(1).Staning with HE: The tissues of normal group and sham-operated group was integrate, but that of I/R were changed with gliocyte hyperplasia and hypertrophia, interstitial edema, cellular edema, gliocyte and neuron edema, neuron necrosis.(2).Immunohistochemistry: There were a few expressions HSP27 and IGF-1 in both normal control group of 5 gerbils and sham operation group of 5 gerbils, and there was no significant difference between the two groups (P>0.05). Compared with normal control group, both of the levels of IGF-1 and HSP27 in neurons and gliacytes of ischemia-reperfusion gerbils were evidently upregulated at the 6th hour, 1st day, 3rd day and 7th day,and peaked at the 3rd day (p < 0.05), then bothdownregulated gradually.Conclusions(1) Expressions of HSP27 protein increased following cerebral ischemia- reperfusion, which is a protective factor to the brain tissue.(2) Expression of IGF-1 was upregulated and can exert neuropro-tective effects following cerebral ischemic reperfusion.(3) There was a link between expression of HSP27 and expression of IGF-1 after brain tissue I/R injury.