Experimental Study Of Freeze-dried And Irradiated Porcine Dural Xenotransplantation

Objective: To observe the effect of xenotransplantation of porcine freeze-dried and irradiated dura mater and develop a new xenogeneous dura mater substitute material. Methods:â‘ . freeze-dried porcine dura mater, irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater were prepared by freeze-dried and(or) irradiated treatment. Biomechanic properties and bacterial growth were detected in vitro. Histologic properties were also observed.â‘¡. 45 rabbits were randomly divided into three groups and transplanted with freeze-dried porcine dura mater (group A), irradiated porcine dura mater (group B) and freeze-dried and irradiated porcine dura mater (group C) respectively. Histologic properties were observed postoperatively(2,6and 12 week) dynamically. Immunologic property was determined postoperatively(3 and 8 week).Results:â‘ Before implantation, the ultimate tensile stress of irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater was markedly greater than that of freeze-dried porcine dura mater (P<0.01), However, there was no significant difference between irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater (P>0.05). The time of resistance to collagenase digestion was longer markedly in irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater than that of freeze-dried porcine dura mater (P<0.05), However, there was no significant difference between irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater (P>0.05). No bacteria was found in irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater but in freeze-dried porcine dura mater.â‘¡Histological results before operation: fibers thickening and fiber density increasing was observed in irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater. At 2 weeks after transplantation, incision has been healing, cellularity of all grafts has increased. Cerebrospinal fluid leakage was not found. At 6 weeks after transplantation, cellularity of all grafts had continually increased. At 12 weeks, covered with cells of all grafts group and the greatest density of group C were observed.â‘¢The contents of lymphocytoxicity showed lower in Group C than that in Group A and B(P<0.05).Conclusion: Freeze-dried and irradiated porcine dura matar is a good substitute for xenogeneous grafts, which can remarkably decrease the antigenecity, with sterility and strong biomechanic properties.