| Objective: To investigate the efficacy of combined use of Morphogenetic Protein (BMP)- Calcium Phosphate Cement (CPC) - freeze-dried dura mater (FDDMA) in clinical segmental bone defects repairing. Methods: 24 experimental rabbits were randomized into 4 groups; 6 rabbits in each group. Except for 3 the right upper limbs in 3 rabbits of them , the rest have made 10 mm segmental bone defects in the radius . Thereinto 3 rabbits with left defects repaired with BMP- CPC - FDDMA were taken as experiment group (12 limbs ); right defects repaired with ARM-CPC-FDDMA as control groups, and another 3 rabbits left defects transplanted no material as blank group, without any treatment in the other sides. The X-ray examinations were done at 1,2,4,6,8,12 week after operation. The Lane-Sandhu evaluated method was applied to determine the quantity of the callus formed in bone defects which displayed on radiograph and each group total relatively data were calculated. The evaluated results were tested by Paired-Samples T Test of software SPSS of statistics. The samples of pathology were done in 2,4,8,12 week after operation, the area of bone samples were analyzed by Leica Qwin system, and relative area in each group of new bone percentages was counted, and its results were tested by One-Way ANOVA test.Result: The result is sameness whether it is in the X-ray or histology. The synthesis evaluating of X-ray results of experiment groups are better than control groups (p<0.01). The analytic results of the new bone area by computer in the histology of experiment groups are better than control groups (4,8,12 week after operation, p<0.05), two groups are seen no difference in second week(p>0.05);Obviously the experiment groups and control groups are better than blank groups (p<0.01). The self contrast of experiment groups and control groups have significant difference in 2,4,8,12 week(P<0.05); The contrast result of blank groups between week two and other times has significant difference (P<0.05), no difference in the 4,8,12 week(P>0.05). Conclusion: 1,The combined matter of BMP-CPC-FDDMA has a good histocompatibillity; 2,The result revealed that the bone defect in the BMP-CPC-FDDMA group had more excellent repair than that seen in the ARM-CPC-FDDMA group. The membrane pipe produced by freeze-dried dura matter from the combined matter of BMP-CPC-FDDMA that has the barrier effect which works the same way as the technology of membranous guiding bone regeneration and prevents parenchyma around from invading the area of bone defect. While the effect of inducing osteogenesis performance from BMP is used the effect of bracket and conduction osteogenesis performance from CPC manpower bone are also used. The BMP, CPC and FDDMA are combined to transplant to repair the bone defect, which cooperate with effect of osteogenesis, thus works effectively to repair the bone defect, and will be an effective method for segmental bone defects repair; 3,BMP has mighty effect on inducing osteogenesis but it will has no good effect if used alone in the area of bone defect. CPC not only is the finer carrier of BMP but also can keep the crevices inferior to membrane. After combined CPC with BMP was transplanted into the membrane, the inducing osteogenesis becomes the primary form in the bone regeneration.
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