The Effect Of Simvastatin On Bone Tissue In Diabetic Rats In Earlier Period

Purpose:To investigate the effect of interventional therapy of simvastatin on diabetic rats,and the changes in bone mineral density (BMD),bone morphology,bone metabolism and bone tissue bone morphogenetic protein-2(BMP-2),as well as serum interleukin-6(IL-6).Method:After one week'adaptive feeding,thirty SPF 4-months-old male SD rats were randomly selected as group A(normal control group) and group B.A is 10 rats,B is 20 rats.The rats of group A inject citrate buffer liquid in abdominal cavity.After the rats of group B has been established the diabetic model by intraperitoneal injection of streptozotocin,it was randomly selected as group B₁(diabetic control group) and group B₂(simvastatin treatmement diabetic group).Group B₁ and B2 both are 10 rats.The rats of group B2 was administered orally simvastatin(5mg.(kg^-1·d^-1)),as well as group A and group B₁ were administered orally 1.5ml normal saline every day.At the end of 14 weeks,the BMD was determined.Then all group rats were killed,bone morphology,serum alkaline phosphatase(ALP),serum tartaric-resistant acid phosphatase(TRACP),and bone tissue BMP-2 gene expresses,as well as serum IL-6 and so on were investigated.Result:BMD:Compare with group A,the group B₁ was significantly decreased(P＜0.01);Compare with group B₁,the group B₂ was increased (P＜0.05).Bone morphology:the trabecular bone in group B₁ has decreased, and compared with group B₁,the group B2 was improved.Bone metabolism index:compared with group B1,the serum TRACP in group B₂ was significantly decreased(P＜0.01),and the serum ALP in group B₂ was increased(P＜0.05).The level of bone tissue BMP-2 gene expresses: compared with group B₁,the group B₂ was increased(P＜0.05).The serum IL-6:compared with group B₁,the group B2 was decreased(P＜0.05).Conclusion:Osteoporosis feature was found in the STZ-induced diabetic rats after 14 weeks.The simvastatin could increase the BMD, improve the bone morphology and bone metabolism,and enhance the bone tissue BMP2-mRNA expression,as well as decrease serum IL-6 in diabetic rats.This can provide experimental basis on simvastatin improving and treating the diabetes complicated with osteoporosis.