| Objective: To investigate the correlation between DC-specific ICAM-3 grabbing nonintegrin expression and Mycobacterium tuberculosis in human.Methods: The peripheral blood mononuclear cells were obtained respectively from 25 patients with tuberculosis, 25 patients with non-tuberculous pneumonia and 25 healthy individuals (M/F:13/12 per group,all aged under 55 years, with both HBsAg and anti-HIV negative), and were cultured in medium with GM-CSF and IL-4. Five days later, expression of CD11c, CD86, HLA-DR and DC-SIGN were detected by flow cytometry. The expression of DC-SIGN mRNA were detected by RT-PCR.The levels of IL-12 and IL-10 in the supernatant were measured by enzyme-linked immunosorbent assay. Proliferation induction of dendritic cells to T lymphocytes was measured by mixed lymphocyte reaction.Results: The expression of CD86 on DCs in the tuberculosis patients [(72.28±10.69)%] was significantly lower than those in the other two groups [(87±16)%, ( 92±6)%] ( F=11.97, P<0.01). In patients with tuberculosis, the expression of DC-SIGN on DCs [(857±8)%] was much higher than the two other groups [(60±28)%, (62±13)%] ( F=8.27, P<0.01). So was DC-SIGN mRNA level (F=3.99, P<0.05). The levels of IL-10 secreted by DCs in the tuberculosis patients [(98±31) ng/L] were significantly higher than those in the other two groups [(74±38) ng/L, (66±27) ng/L] (F=4.19, P<0.05). Mixed lymphocyte reactions showed lower potency of proliferation induction of DCs to T lymphocytes in the tuberculosis patients (1858±628) than the other two groups (3066±1389), (3383±1163) (F=7.92, P<0.01). Expression of CD11c and HLA-DR on DCs and IL-12 levels were not statistically significant among the three groups (P>0.05).Conclusion: The decreased DCs maturity, elevated IL-10 secretion by DCs, decreased proliferation induction of DCs to T lymphocytes and impaired immune response may due to overexpression of DC-SIGN in patients with tuberculosis.
|
PDF Full Text Request