The Expression And Meaningful Of S100A4 And UPA Protein In Colorectal Carcinoma

Objective: The main purpose of this study was to study S100A4 in colorectal cancer and the expression of uPA protein and to analyze their expression and biological behavior of colorectal cancer the relationship between the Sl00A4 explore and uPA as a molecular marker to assess tumor invasion and metastatic potential may be and colorectal cancer for further research and clinical treatment in accordance with the provision of pathology.Methods: This study used immunohistochemistry to be fast MaxVisionTM immunohistochemistry combined detection of protein levels in colorectal cancer in S100A4, uPA expression, using known positive sections as controls.(1) of this study was to select the Yan'an University Hospital in January 2004 -2005 during the trip in January after surgical resection and pathological diagnosis confirmed 63 cases of patients with colorectal cancer, the collection of pathological specimens archived wax blocks, at the same time to select 20 cases of normal mucosa as a control. 19 cases of colorectal adenoma, both mild and moderate dysplasia.(2) for each wax block sections 3, thick 5μm. One of the sections for HE staining, conventional pathological observation, and the other two sections by immunohistochemical methods (MaxVisionTM Act) for S100A4, uPA expression level of detection. Analysis of S100A4, uPA in colorectal cancer tissue and normal colorectal tissues differences in patients with colorectal cancer with age, sex, tumor site, gross type, histological type, degree of differentiation, Dukes stage, lymph node and liver metastases relationship.The results: (1) S100A4 protein in normal colon tissues did not express, in colorectal adenomas for the 3 / 19 cases (15.8%) positive, in colorectal cancer tissues for 25/63 cases (60.3%) positive , normal mucosa and adenoma group P = 0.068, not statistically significant, colorectal cancer group and normal mucosa group (p = 0.001), colorectal adenoma group (p = 0.032) compared P <0.05, statistically significance. S100A4 protein in the Dukes Staging A period of five cases were negative expression; B period expressed as 5 / 25 cases (20%) positive; C expression for the period 9 / 16 cases (56.3%) positive; D view of expression 11/17 cases (64.7%) positive, the results of P = 0.003 there is significant difference. differences. Distant metastasis in colorectal cancer cases in the 8 / 11 cases (72.7%) S100A4 protein expression was, in the absence of distant metastasis in colorectal cancer cases in 17/52 patients (32.7%) S100A4 protein expression positive, the results of p = 0.017, p <0.05 significant difference exists.(2) uPA protein in normal tissues did not express the large intestine, the expression in colorectal adenoma 2 / 19 cases (10.5%), in colorectal cancer tissues for 46/63 cases (73.0%), in the normal mucosa group adenoma group and P = 0.141, not statistically significant, colorectal cancer group and normal mucosa group (p = 0.000), colorectal adenoma group (p = 0.000) compared, P <0.01 statistically significant results. uPA protein in the Dukes Staging A period expressed as 2 / 5 cases (40.0%) positive; B expression for the period 15/35 patients (42.9%) positive; C expression for the period 14/16 patients (87.5%) positive; D period of expression of 15/17 cases (88.2%) positive, the results of p = 0.033 there is significant difference. differences. Distant metastasis in colorectal cancer cases in 11/11 patients (100.0%) uPA protein expression was, in the absence of distant metastasis in colorectal cancer cases in 35/52 patients (67.3%) uPA protein expression positive, the results of p = 0.022, P <0.05 significant difference exists.(3) in colorectal cancer, S100A4 protein expression in 38 cases of negative, uPA protein expression was negative in 16 cases, 22 cases of positive expression; S100A4 protein expression in 25 cases of positive, uPA protein expression of 1-negative cases, positive expression of for 24 cases. The results of analysis using Spearman rank correlation (r = 0.420, p = 0.001), positive correlation between the meaning of existence.Conclusions: (1) S100A4 protein in colorectal adenocarcinoma was significantly higher than expression in colorectal adenoma and normal mucosa tissues, its expression and colorectal cancer Dukes stage, lymph node metastasis and distant related. S100A4 over-expression prompted the promotion of colorectal adenocarcinoma and metastatic infiltration.(2) uPA protein in colorectal adenocarcinoma was significantly higher than expression in colorectal adenoma and normal mucosa tissues, its expression and colorectal cancer Dukes stage, lymph node metastasis and distant related. Over-expression of uPA prompted the promotion of colorectal adenocarcinoma and metastatic infiltration.(3) S100A4 and uPA protein expression was positively correlated, suggesting that colorectal cancer tissue uPA protein S100A4 interaction with the promotion of colorectal adenocarcinoma and metastatic infiltration.