The Therapeutic Study Of Transplantation Of Endothelial Progeniton Cells And Atorvastatin In Acute Lung Injury Of Rats

Objective:To investigate the reparative functions of endothelial progenitor cells(EPCs) in rats'model of Acute lung injure(ALI). Compari- son of the therapeutic effects of transplantation of EPCs and statin and the therapeutic alliance.Methods:Circulating EPCs were obtained from human cord blood using Ficoll centrifugation.One week after culturing,EPCs were labed by BrdU. ALI was induced in SD rats by LPS.All animals were randomized into five groups(Group I,injected with LPS and saline;Group II,injected with LPS and treated by transplantation of EPCs;Group III,injected with LPS and treated by Lipitor;Group IV,injected with LPS ,treated by transplantation of EPCs and Lipitor;Group V,injected saline.Degrees of lung injury were assessed 2 days later.Expression of inducible nitric oxide synthase(iNOS) in pulmonary artery was measured by ELISA.Results:The BrdU immunohistochemistry analysis was positive in group III,IV.Endothelial dysfunction in the pulmonary artery was signify- cantly attenuated in rats treated with EPCs.Expression of iNOS was suppressed in the pulmonary artery of EPC-treated animals.Infiltration of leukocytes in the lung parenchyma was significantly reduced after EPC tranplantation.EPCs also decreased water content,hyaline membrane forma- tion,and hemorrhage in lungs.Conclusion:Transplantation of EPCs can preserves pulmonary endo- thelial function and maintains the integrity of pulmonary alveolar-capillary barrier.The effect of combined treatment of EPCs and statin is the best.Transplantation of EPCs can be a cell-based,endothelium-targeted therapeutic strategy for prevention and treatment of ALI/ARDS.