The Effect Of Acupuncture And Exercise Intervention In Different Combinate Order Treat The Bilirubin Encephalopathy's Rat In The Cerebral Cortex BDNF Expression

Purpose: To explore the effects of Acupuncture and exercise in the different order of combination treatment interventions rat model of bilirubin encephalopathy in rat cerebral cortex BDNF expression, for the treatment of bilirubin encephalopathy in accordance with the provision of laboratory.Method: Select 20 young SD rats were randomly divided into 4 groups: normal group, model control group, exercise after the first acupuncture group (AE), acupuncture group after the first exercise (EA). To one of normal saline by intraperitoneal injection line, and the remaining three groups are the rule of law model bilirubin bilirubin encephalopathy model. Model untreated control group. Model in the treatment group the day after the success of treatment: Acupuncture select Top bilateral temporal pre-slash, exercise therapy training method used, each 40-minute treatment, acupuncture and exercise the 20 minutes, 1 second / day , 1 week course of treatment. After the treatment, in accordance with the in situ method based on taking the whole brain tissue, the block repair organizations, take part of the cerebral cortex between areas, by the former fixed dehydration→rinsing→immersion embedding, polymerization→biopsy, Immunohistochemistry, such as sample preparation process at universal electron microscopy, using Motic Images Advanced 3.0 X-ray film, Motic Images Advanced6.0 medical image analysis system for measurement of immunohistochemical-positive cells, and the statistics of each group separately in cerebral cortex of BDNF-positive cells. Comparison of the cerebral cortex in each group the number of BDNF positive cells, to do statistical analysis.Result: (1) normal rats does not appear insensitive, prone, convulsions and other phenomena; model group rats showed a bit too slow, prone position, such as convulsions. Cerebral cortex were observed dye visible by immunohistochemistry of BDNF positive cells in yellow, model control group and treatment group bilirubin deposition can be seen. (2)①model group (180.40±6.88) than the normal group (89.95±6.52) BDNF-positive cells have a very significant difference (P <0.01);②first acupuncture group after exercise (127.81±3.13) and normal group ( 89.95±6.52) compared have very significant difference (P <0.01);③first acupuncture group after exercise (127.81±3.13) with the model group (1.07±0.03) compared have very significant difference (P <0.01);④first acupuncture group after exercise (136.59±10.52) and normal group (89.95±6.52) more significant difference P <0.01);⑤first acupuncture group after exercise (136.59±10.52) with the model group (180.40±6.88) compared there is a very significant difference (P <0.01)⑥first acupuncture group after exercise (127.81±3.13) after exercise with the first acupuncture group (136.59±10.52) compared there is a very significant difference (P <0.01).Conclusion: After the treatment group after treatment of rats improved over the previous response, a flexible activity, brain-derived neurotrophic factor (BDNF)-positive cells lower than the model group, but still higher than the normal group. Acupuncture and exercise therapy can promote the recovery of young rats to improve brain function, so that the positive expression of BDNF declined, suggesting that effective treatment. The order of two different combinations of intervention contrast effects were significantly different (P <0.01), exercise group after the first acupuncture positive expression of BDNF is less than the first acupuncture group after exercise, suggesting that more is better than AE Group EA group efficacy.