Histopathological Changes Of Articular Cartilage In Experimental Limb Applicating Ilizarov Distraction Technique

Objective To ascertain the effect of change traction on articular cartilage by utilizing Ilizarov distraction technique.Methods Experiment at 18 dogs left ankle joints were fitted with Ilizarov external fixator,the use of 1mm/d of the stretch speed,firstly create a varus deformity,three weeks time,to the ankle varus 15°,taken X-ray film confirmed after the cessation of stretch three weeks,and then the same speed(1mm/d),to correct deformities,three weeks time.To observe the morphological changes of the medial articular cartilage by adequating health medial articular cartilage and articular cartilage of medial foot in the third week,the seventh week and ninth week of the experiment and HE staining. Through immunohistochemistry,application software Image-Pro Plus 6.0 image to obtain the value of statistics to deal with SPSS13.0.Using transmission electron microscopy to observe microstructure of cartilage.Results 1,Generally,we can see the original blue and white,shiny,no cracks in the articular surface of the cartilage after the stress increases gradually yellow,surface roughness,cracks appear;when the pressure decreases,the yellowing,rough,the color of the fracture restore gradually and become shiny.2,the shiny smooth surface can be seen under a light microscope,formation,cell distribution,tidy,dear the level of cartilage at the articular surface stress increases,the surface roughness changes, defects,disordered cells,uneven dyeing;when the articular surface of the pressure gradually decreased,the cartilage gradually repair and the surface of cells at the surface appear only disorder.3,immunohistochemical observation can be seen throughout the observation period,cartilage cells are typeⅡcollagen expression and expression after 3 weeks gradually weakening,when the seventh week begin to strong gradually.4,electron microscopy shows that when stress increases the articular surface,the cartilage cells became flat,the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders;and when stress decreases the articular surface,cartilage cells gradually returned normal,cytoplasm in the endoplasmic reticulum,Golgi body gradually restore quantity;collagen fibers with a gradual rules.Conclusion Traction at ilizarov stretch technology,when the pressure between the cartilage surface of the ankle joint increases,the articular cartilage necrosis occurs and when the stress between the articular cartilage surface decreases,the articular cartilage regeneration can be able to restore.