Studies On Detecting Aflatoxin M₁ In Milk And Effects Of Aflatoxin In Feed On Milk Quality

Aflatoxin has been proved a reason resulting in cancer in human being. Aflatoxin M1 is the metabolize production of aflatoxin B1.Aflatoxin M1 in milk comes from animals fed feeds have been polluted by aflatoxin B1.So the effort to prevent and dispose aflatoxin B1 is very important.Although many studies have been carried out inside and outside,so far there isn't an ideal means applied in industry.At present the best measure to reduce the harm of aflatoxin is to detect aflatoxin in food in time.Recently,high sensitive instrument analysis means and rapid effective immunity means have been applied in detection.Aflatoxin M1 in milk are extracted and separated by immunoaffinity column.Then the column in washed with methanol water(10:90) twice and the concentrated aflatoxin M1 are eluted with methanol water(80:20).The aflatoxin M1 is determined by fluorometer with bromine.Test portions and spiked at 0.1,0.5,1.0μg/kg, recoveries are 89.6%～96.1%,variable coefficient are 0.52%～5.8%,the lowest.It is 30 minutes for analysis one sample.The average content of aflatoxin Blin silage,coarse fodder and blend diet is 8.28μg/kg, 3.11μg/kg,and 6.56μg/kg.respectively,the pollution is light.50 samples have been detected are all accorded with the limited standard in our country,the maximum of positive is 0.203μg/kg,the minimum is 0.003μg/kg,and the average value is 0.089μg/kg.There is relationship between aflatoxin M1 in milk and aflatoxin in feed.There is about 1.3% aflatoxin in milk comes from feed.