Expression Of Synaptophysin And α-Synuclein In The Brains Of Parkinson Disease Rats With Trihexyphenidyl

Backgroud:Parkinson's disease(PD) is the most common,progressive neurodegenerative disorders.PD patients suffer from static tremor,cogwheel rigidity,bradykinesia and postural instability.Yet,recent studies have found that non-motor symptoms(NMS) including neuropsychiatric impairments,autonomic dysfunction,sleep disorders, gastrointestinal symptoms,and sensory disorders frequently complicated the course of the illness.About 30%PD patients suffer from Parkinson Disease Dementia(PDD). The trihexyphenidyl(Artane) is one of the anticholinergic drugs for the treatment of Parkinson's disease.It is considered more effective for the symptoms of tremor and rigidity without altering bradykinesia.The adverse effects are frequent and include dry mouth,urinary retention,constipation,nausea and impaired sweating.The central side effects such as decreased memory,confusion,and psychosis with hallucinations are also frequent in clinical practice.But we found different patient has different reaction to trihexyphenidyl.Some produced memory impaired within 1 month and some without obviously memory lost even in 5 to 10 year.we can suppose that there are other mechanisms expect counting to choline of the cognitive disorder induced by trihexyphenidyl.Objective: During the study,we try to find the relations between the cognitive function disorder and different expression of the synaptophysin andα-synuclein in the rat's brains and make sure the pathology of the cognitive function disorder with trihexyphenidyl.Methods:1.30 rats were randomly chosen from 90 male Wistar rats and divided into blank group and sham operated group.2.Other 60 rats were established by the 2 points injections of 6-OHDA into the corpus stiatum while the sham operated group rats were injected of the equal doses normal sodium.They were induced by emetomorphine 2 weeks after injection and 36 PD models were established.30 models were randomly chosen and divided into PD model group and medicine intervention group.3.The baseline was established after tested with the Morri's water maze for memory and learning function.4.The PD rats interfered by trihexyphenidyl were lavaged and began to take trihexyphenidyl after 4 weeks.Other rats were lavaged and also began to take the equal doses normal sodium.5.After 3 months all the rats were tested by Morri's water maze for memory and learning function again.6.After sacrificed,we made them to be paraffin sections.Frontal lobe and brain stem microstructures were observed by an optic microscope after being stained.The expressions of synaptophysin andα-synuclein were detected by an immunohistochemical method.7.Statistic Manual:The data were analyzed using SPSS13.0 for Windows (Chicago,IL.) and t-test.The results are expressed wit Mean±standard deviation((?) +S).A level of significance of P＜0.01 was used.Results:Different expression levels were presented of synaptophysin andα-synuclein between 4 groups in the frontal lobe and brain stem.The number of synaptophysin imunoreactive neurons in frontal lobe of medicine intervention group was obviously reduced compared with model rats(P＜0.01).The number ofα-synuclein imunoreacive in frontal lobe was increased compared with model rats(P＜0.01).Conclusion:Taking trihexyphenidyl can be result in memory disorder of the rats.The increase of theα-synuclein and the decrease of synaptophysin in the frontal lobe were correlated with the memory impairment of the rats with trihexyphenidyl.