Expression And Clinical Significance Of SKP-2 And PTEN In Pathological Scars

ObjectiveTo study the contribution of SKP-2 (S-phase kinase associated protein 2,) and PTEN (phosphatase and tensin homology deleted on chromosome 10) in pathological scars by investigating the expression difference and correlation between SKP-2 and PTEN in keliods, hypertrophic scars, normal scars and normal skins.MethodsThe samples are collected from 62 patients ,in which there are 11 with keloids, 18 with hypertrophic scars (in 1 year after injury), 17 with normal scars and 16 with normal skins to contrast. Immunohistochemical staining (SP method) was used to detect the expression of SKP-2 and PTEN.Three sections are incised by serial section from each sample.The third section is used for negative control with PBS as the first antibody. The purpose is comparing the expression difference of SKP-2 and PTEN in keloids,hypertrophic scars,nomar scars,and nomar skins.Results1. The expression levels of SKP-2 are significantly higher in keloids and hypertrophic scars than in normal scars and normal skins(P<0.05),while the expression levels of PTEN are significantly lower in keloids and hypertrophic scars than in normal scars and normal skins(P<0.05).2.The differences of SKP-2 expression and PTEN expression between keloids and hypertrophic scars are not significant (P>0. 05) . And the differences of SKP-2 expression and PTEN expression between normal skins and normal scars are not significant( P>0. 05)3.There is a negative corralation between expression of SKP-2 and PTEN in the four kinds of skin tissues Conclusion1.High expression of SKP-2 and low expression of PTEN in their tissues may play important roles in the pathogenesis of keloids and hypertrophic scars2.There is a negative corralation between expression of SKP-2 and PTEN in the four kinds of tissues.