The Effect Of Inhibition Of Expressing TF For IBMIR In Islet Cell Transplantation

Objective: Antisense RNA was constructed to inhibit expression of TF in porcine islet cell,then The Effect of inhibition of Expressing TF in islet cell was observed for IBMIR after transplantation.Methods:5 antisense RNA were designed to transfect porcine islet cell with antisense technology,then the semiquantitative reverse transcription-PCR and realtime-quantitative reverse transcription-PCR were applied to select the best Antisense RNA for TF silencing; the indexes were detected in vitro model of IBMIR in the porcine islet cell after TF silencing, to known the level of development of IBMIR.Result:The oligoTF-1006,oligoTF-1009,oligoTF-114 which had effect of silence were selected by PCR detection;the realtime- quantitative PCR showed that the results of TF-mRNA seliencing after 4 days by transfection of these three Antisense RNA in islet cells,which are 71.95%,59.63%,19.05% respectively; the weight of blood clot was lighter when the islet cells which were transfected 4days ago had been added in IBMIR models in vitro according to PCR,and the blood indexes showed that the development of IBMIR in these tthree groups were lighter than ones in blank transfection group.Conclusion: There is a relation between TF which is the important factor to start blood cloting path and IBMIR after replantaion of islet cells,and antisense RNA can inhibit the espression of TF and reduce the development of IBMIR after replantaion of islet cells.This method can be used to lower the lost of grafts and increase the survival rate of islet cells clinically in the future.