Antitumor Effect And Mechanism Of The Partial Purified Scorpion Venom On Human Breast Cancer Cell Line MCF-7 In Vitro

Cancer is a serious threat to human life and safety of one of the diseases. To seek efficient and low toxicity of anticancer drμgs and treatment of the scientific community has been on the hot spots. Scorpion venom is a biological toxin, because of its wide range of physiological effects closely investigated. This paper aims to study the destruction about scorpion venom on human breast cancer cell line MCF-7 and sensitive cell line, and their mechanisms were discussed for the development of scorpion toxin of anti-tumor clinical applications experimental basis.Objective: detected growth inhibition of using the extractive of scorpion venom on human breast cancer cell line MCF-7, and investigated the mechanism.Methods: bolting the sensitive cell line in hepatoma cell SMMC-7721,Hela cell and breast cancer cell MCF-7 by MTT and IMF; detecting the apoptosis-related proteins expressed levels of caspase-3 and Bcl-2 proteins with ICC; observed the effect on cell cycle by FCM; detecting the cell cycle-related protein expressed levels of cyclinD1 with western blotting. Results: MTT method displayed that the inhibition on 7721 and MCF-7 by using the 600μg/ml extractive of scorpion venom, compared with the negative group(p<0.05);IMF method displayed that the sensitive to scorpion venom of 7721 cell was better than Hela cell after drug intervention; ICC method displayed that apoptosis protein caspase-3 increased and anti-apoptosis protein Bcl-2 decreased after drug intervention; FCM displayed that the amount of S period of time decreased, the amount of G1 period time increased after drug intervention, compared with the negative group(p<0.05); western blotting displayed that the expression of cell cycle-related protein cyclinD1 decreased after drug intervention.Conclusion: the extractive of scorpion venom inhibited the growth of human breast cancer MCF-7, and induced apoptosis in MCF-7 cell, the mechanism maybe related to G1 and S period of time, there was a significant value in treating human breast cancer.