Expression Of MMP-3 And NF-κBp65 In Colonic Mucosa Of Rats With UC And Its Significance

Objective: To detect expression of MMP-3 and NF-κBp65 in colonic mucosa of rats with UC, to explore the role of them in the carcinogenesis of UC, and to provide a novel insight and means for clinical diagnosis and treatment, as well as prevention in UC.Methods: Forty clean and mature rats were studied in our experiment, half of them are the male, twenty rats were used as model group at random among them, adopted compound method (2, 4-dinitrochlorobenzene plus acetic acid ) to make model of rats with cell immunoreactivity UC, the other 20 normal rats were used as control group. A week and two weeks after the model was finished, cut the belly open and acquired biopsy specimens from mucosa of model group and control group, 10 portions in each group respectively, all the specimens were taken conventional pathological section and were diagnosed by two skilled pathologists. Expression of MMP-3 and NF-κBp65 in colonic mucosa of 20 rats in model group and 20 rats in control group were detected by immunohistochemical Elivision method, and analyzed their relationship with the seriousness of disease. SPSS version 14.0 for windows software was used for the statistical analysis, usingχ~2 and Spearman relation analysis in qualitative data, SNK-q test of One-way ANOVA in quantitative data, P value less than 0.05 were considered statistically significant.Results: 1,20 rats of model group began to occur the common symptoms of UC such as diarrhea,filthy crissum,stench stool, and appeared to mucus and purulent stool,bloody stool or thin and noncoagulative stool; hyperemia,hydrops,anobrosis,necrosis of colonic mucosa,thick intestinal parietes and ulceration was seen from gross appearance of specimen. All of them proved to accord with the standard of UC by comprehensive information from clinic symptoms,gross appearance and pathological diagnosis. The successful rate of making model is 100%. 2,The positive expression rate of MMP-3 in colonic mucosa of model group and control group were 90% (18/20) and 30% (6/20) respectively, expression of MMP-3 in colonic epithelium of model group was significantly higher than that of control group, the difference has statistical significance(P<0.01). 3,The expression of MMP-3 in three grades(mild,moderate,wild) of rats with UC were 18.75±14.57,51.83±20.37,83.0±29.56;the mild copared to the moderate, P value is less than 0.05, also the wild copared to the moderate and the mild , P value is less than 0.05, too. 4,The positive expression rate of NF-κBp65 in colonic mucosa of model group was80 % (16/20), and was25 % (5/20) in control group, there was significantly difference between them (P=0.004,P<0.01) .5,The expression of NF-κBp65 in three grades(mild,moderate,wild) of rats with UC were 8.5±6.86,40.17±13.15,67.60±27.06; there was statistical difference among them(P<0.05). 6,The expression of NF-κBp65 in colonic mucosa of rats with UC has a positive correlation with that of NF-κBp65 (r_s =0.917, P<0.01) .Conclusion: 1. MMP-3 and NF-κBp65 in colonic mucosa of rats with UC were obviously increased, and were a positive correlation with the seriousness of disease, which shows that they may be play a important role in the occurrence and development of UC. 2. Expression of MMP-3 and NF-κBp65 closely related in the UC colon mucosa, and they have a significantly positive correlation. The MMP-3 may be promote the NF-κBp 65 expressions.