The Effects Of Oxymatrine Injection On Expression Of Cytokines And Nuclear Factor-κBp65 In Colonic Intestinal Mucosa Of Ulcerative Colitis Rats

Objective:To establish the ulcerative colitis(UC) model rats induced by 2, 4, 6-trinitro-benzene-sulfonic acid (TNBS).To detect the levels of interleukin-2(IL-2) and interleukin-10(IL-10) and expression of nuclear factor-kappa Bp65 (NF-κBp65) in UC rats colonic intestinal mucosa after the treatment for UC rats with Oxymatrine (OMT) Injection. And to evaluate the efficacy, to study and research the anti-inflammatory mechanism of Oxymatrine Injection.Methods:40 male rats were randomized into the following groups (n=10 per group): normal control, experimental colitis, experimental colitis with Mesalazine, experimental colitis with OMT treatment. Ulcerative colitis was induced by the mixing of TNBS and 50% alcohol in each group except for normal control. The rats in experimental colitis were injected 0.9% saline in an equal volume as OMT treatment for 15 days. The rats in Mesalazine group were lavaged by Mesalazine at the dosage of 0.42g*kg-1 *d-1 for 15days. And the rats in OMT treatment were intramuscular injected with OMT at the dosage of 63mg*kg-1 *d-1 for 15days.the rats in normal control drank water and feed food normally. Diarrhea and bloody stool as well as colonic mucosa damage observed. After stainning with hematoxylineosin (HE), the degrees of inflammation in colonic mucosa were graded with microscopic observation. Retained in Glutaral for few hours, ultramicro-pathology change of UC rats colonic mucosa was observed by electron microscope. The levels of IL-2 and IL-10 were determined by ELISA, and NF-κBp65 activity were detected by immunohistochemistry method Results:①Oedema, anabrosis, hyperemia, and cicatricle was observed in the colonic mucosa of experimental colitis. And the the ulcerative colitis model of rats was made successfully.②The inflammatory symptoms (diarrhea, bloody purulent stool, et al)of colonic mucosa in OMT-treated group were significantly improved.③Compared with experimental colitis, the colonic mucosa damage index as well as the degrees of inflammation of colonic mucosa in OMT-treated group was significantly decreased(p<0.01). And ultramicro-pathology damage of colonic mucosa in OMT-treated group was relieved obviously.④Compared with normal control, the level of IL-2 in the experimental colitis rats was increased(p<0.01). Compared with experimental colitis , the level of IL-2 in OMT-treated group was decreased(p<0.05).⑤Compared with normal control, the level of IL-10 in the experimental colitis rats was reduced(p<0.01). Compared with experimental colitis, the level of IL-10 in OMT-treated group was increased(p<0.05).⑥Compared with normal control, there was increased expression of NF-κBp65 in the experimental colitis rats(p<0.01). Compared with experimental colitis, the expression of NF-κBp65 in OMT-treated group was significantly reduced(p<0.01).⑦And the expression of NF-κBp65, IL-2 and IL-10 in the OMT-treated group was similar(no statistical difference, p>0.05) as Mesalazine-treated group.Conclusion:Oxymatrine Injection is effective obviously to treat ulcerative colitis in rats. With the administration of Oxymatrine Injection, the clinical symptom of ulcerative colitis rats was relieved rapidly, the degrees of inflammation as well as colon injury were alleviated, and ultramicro-pathology damage of colonic mucosa was restored. Inhibiting the expression of NF-κBp65, IL-2 and promoting the expression of IL-10 may be the anti-inflammatory mechanism of Oxymatrine Injection.