The Study Of Expression Of AngⅡ And Its Receptor 1 Proteinsin Ovarian Cancer

Objective:1.To investigate the expression of Ang II and its receptor 1,VEGF,CD34 in normal ovarian tissues and ovarian tumor tissues,and analyze the correlations of the above indexes expression with several clinicopathologic characteristics.2.To detect the mRNA expression of AT₁R in ovarian cancer cell lines SKOV3 and ES2.3.To detect the mRNA expression of AT₁R in normal ovarian surface epithelium and ovarian tumor tissues,and analyze the correlation with several clinicopathologic characteristics.Method:1.Expression of Ang II and its receptor 1,VEGF,CD34 were detected in 20 normal tissues,20 benignin tissues,20 bordline tissues and 60 ovarian cancer patients by using immunohistochemistry. .2.RT-PCR was used to detect the mRNA expression of AT₁R in ovarian cancer cell lines SKOV3 and ES2.3.RT-PCR was used to measure the mRNA expression of AT₁R in 6 normal ovarian surface epithelium and 30 ovarian cancer patients.Result:1.Angâ…¡was not expressed on the surface epithelium of the normal ovary,The positive expression of Angâ…¡was detected in benignin tissues,bordline tissues and epithelial ovarian cancer patients,and was localized both on themembrane and in the cytoplasm of tumor cells.The positive rate of Angâ…¡in benignin tissues,bordline tissues and epithelial ovarian cancer patients were 25%,60%,80%respectively.The expression of Angâ…¡was significantly up-regulated in epithelial ovarian cancer compared to bordline tissues and benignin tissues (p<0.05);so did bordline tissues compared to benignin tissues(p<0.05).2..AT₁R was not expressed on the surface epithelium of the normal ovary,The positive expression of AT₁R was detected in benignin tissues,bordline tissues and epithelial ovarian cancer patients,and was localized both on themembrane and in the cytoplasm of tumor cells.The positive rate of AT₁R in benignin tissues, bordline tissues and epithelial ovarian cancer patients were 25%,60%,80% respectively.The expression of AT₁Rwas significantly up-regulated in epithelial ovarian cancer compared to bordline tissues and benignin tissues(p<0.05);so did bordline tissues compared to benignin tissues(p<0.05).3.VEGF was expressed in normal ovary,benignin tissues,bordline tissues and epithelial ovarian cancer patients,and was localized in the cytoplasm of tumor cells.The positive rate of VEGF in normal ovary,benignin tissues,bordline tissues and epithelial ovarian cancer patients were 5%,15%,25%,43.33%respectively.The expression of VEGF was significantly up-regulated in epithelial ovarian cancer compared to normal ovary,bordline tissues and benignin tissues(p<0.05);so did bordline tissues compared to normal ovary and benignin tissues(p<0.05).in contrast,The expression of VEGF was not significantly up-regulated in benignin tissues compared to normal ovary(p>0.05).4.CD34 was expressed in normal ovary,benignin tissues,bordline tissues and epithelial ovarian cancer patients,and was localized in the cytoplasm of vascular endothelial cells.The MVD in normal ovary,benignin tissues,bordline tissues and epithelial ovarian cancer patients were 7.38±0.86,8.43±1.05,10.57±1.24,14.88±1.99 respectively.The MVD was significantly up-regulated in epithelial ovarian cancer compared to normal ovary,bordline tissues and benignin tissues(p<0.05);so did bordline tissues compared to normal ovary (p<0.05).The MVD was not significantly up-regulated in bordline tissues compared to normal ovary and benignin tissues(p>0.05).5.In epithelial ovarian cancer,the expression of Angâ…¡,AT₁R,VEGF and the MVD were not related to the age,histological type,FIGO stage,and so on(p>0.05). the expression of AT₁R,VEGF and the MVD were related to differentiation (p<0.05).6.In epithelial ovarian cancer,the expression of Angâ…¡was positively related to AT₁R,VEGF(P<0.01)and so did the MVD(P<0.05);the expression of AT₁R was positively related to VEGF and the MVD(P<0.05);the expression of VEGF was positively related to the MVD(P<0.05). 7.The prognosis of epithelial ovarian cancer patients who had negative,weakly positive expression for Ang IK AT₁R,VEGF was much more better than that who had strongly positive expression;The prognosis of patients whose MVD...