| IntroductionGrowth and metastatic dissemination of solid tumors requires vascular support for nutritive, oxygen supply and removing the wastes. Tumor angiogenesis is a complex process that comprises the proliferation , motility and extracellular matices, it is associate with several angiogenesis factors . The action of Vascular endothelium growth factor (VEGF) is the most important. VEGF acts via two identified receptors, VEGF - R1 - Flt1 and VEGF - R2 - KDR/Flkl. KDR plays an important role in angiogenesis. Angiogenesis is correlated with tumor prognosis , it may be an important index for tumor metastatic and prognosis. Microvessel density ( MVD) can measured the number of angiogenesis.The aim of our study was to investigate the expression of VEGF, KDR and MVD in epithelial serumal ovarian cancer and the role of them in tumor angiogenesis, so that we can discuss their action in the growth, metastatic and prognosis of Ovarian Serous Carcinoma (OSC).Material and method1. CaseA total of 56 epithelial serumal ovarian cancer and 8 normal ovarian tissues were collected at the department of gynecologic in the First and Second Affiliated Clinical Hospital of China medical University from Mar 2000 to May 2001 after surgeon . 56 tumor tissues were di-vided into 3 groups; 1. 9 epithelial serumal ovarian benign tumors; 2. 9 epithelial serumal ovarian borderline tumors; 3. 38 OSC. All samples were formalin - fixed and pararfin - embedded. Samples were reviewed by two pathologists to confirm histological diagnosis. According to the classification of FIGO (1986 ) . 38 OSC patients consisted of 7 stage I , 5 stage II , 25 stage III, and 1 stage IV patients.2. ImmunohistochemistryImmunohistochemistry of the paraffin embedded sections was performed by S - P method. Briefly 4 - jxm sections were depraffinized and they were treated with 0. 3% hydrogen peroxide and incubated with 10% normal goat serum to block non - specific binging. The sections were then incubated with the first antibody at 4 temperature for a night. They were then washed in Phosphate -buffered saline (PBS) and exposed to the second antibody at 37 Tl temperature for 20 minutes. Then they were treated with streptordin peroxidase also at 37 temperature for 20 minutes. The negative control was used with PBS of the first antibody.3. Analysis of results3. 1 The results of immunostaining were classified as negative ( - ) when there were no positive cells, weakly positive ( + ) when the staining was faint or the positive cells were 50% or less, and strongly positive ( + +) when the number of intensely stained cells was greater than 50%.3.2 MVDAfter stained by CDM antibody, sections were examined under low power {100 x) to indentify the region of highest vessel density. In each section, the five most vascular areas were chosen. A 200 x field in each of these five regions was counted, and the average counts ofthe five fields were recorded. Large vessels with thick muscular walls and large vessels with lumina greater than approximately eight red blood cells were excluded from the count. Single cells or cell dusters were counted. These data will be referred to as MVD.4. Statistical analysisAll data were analyzed by SAS statistic software. The student t test, Fishers exact probability et al were used.ResultPositive immunostaining for VEGF ^ KDR was detected in 92.1% and 84. 2% of epithelial serumal ovarian cancer ,44.4% and 33.3% of epithelialserumal ovarian broderline tumor, 22. 2% and 22. 2% of epithelial serumal ovarian benign tumor respectively. But VEGF was not detected in normal ovarian tissue, KDR was detected in 11. 1 % normal ovarian tissue. MVD was 36. 5 5.2 /HP and 36. 8 5. 3/HP in epithelial serumal ovarian cancer positive for VEGF and KDR while it was 25. 5 3. 2/HP and 29.6 4. 6/HP in epithelial serumal ovarian cancer negative for VEGF and KDR respectively ( they are all P < 0.001). Positive KDR immunostaining was observed more frequently in the advanced stage of the disease (P... |
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